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- PDB-1tlf: UNPRECEDENTED QUATERNARY STRUCTURE OF E. COLI LAC REPRESSOR CORE ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1tlf | ||||||
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Title | UNPRECEDENTED QUATERNARY STRUCTURE OF E. COLI LAC REPRESSOR CORE TETRAMER: IMPLICATIONS FOR DNA LOOPING | ||||||
![]() | LAC REPRESSOR | ||||||
![]() | TRANSCRIPTION REGULATION | ||||||
Function / homology | ![]() DNA-binding transcription repressor activity / cis-regulatory region sequence-specific DNA binding / transcription cis-regulatory region binding / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / regulation of DNA-templated transcription / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Friedman, A.M. / Fischmann, T.O. / Steitz, T.A. | ||||||
![]() | ![]() Title: Crystal structure of lac repressor core tetramer and its implications for DNA looping. Authors: Friedman, A.M. / Fischmann, T.O. / Steitz, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 243.2 KB | Display | ![]() |
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PDB format | ![]() | 199.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 426.2 KB | Display | ![]() |
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Full document | ![]() | 476.9 KB | Display | |
Data in XML | ![]() | 30.6 KB | Display | |
Data in CIF | ![]() | 45.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO A 284 / 2: CIS PROLINE - PRO B 284 / 3: CIS PROLINE - PRO C 284 / 4: CIS PROLINE - PRO C 332 / 5: CIS PROLINE - PRO D 284 |
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Components
#1: Protein | Mass: 32154.693 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Chemical | ChemComp-EMC / #3: Sugar | ChemComp-IPT / Nonpolymer details | THE MOLECULE WAS CRYSTALLIZED AS A COMPLEX WITH ISOPROPYL-BETA-D-THIOGLACTOPYRANOSIDE AND THE ETHYL ...THE MOLECULE WAS CRYSTALLIZ | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.99 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Method: other | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Resolution: 2.6→8 Å / Num. obs: 37549 / % possible obs: 97.5 % / Observed criterion σ(I): 2 |
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Processing
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Refinement | Rfactor Rwork: 0.222 / Rfactor obs: 0.222 / Highest resolution: 2.6 Å Details: THE C1 AND HG ATOMS OF THE EMC HET GROUP ARE CHEMICALLY BOUND. THE BOND DISTANCE BETWEEN THEM IS GREATER THAN EXPECTED DUE TO THE LACK OF BONDING RESTRAINT IN THE REFINEMENT AND LIMITED RESOLUTION. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.6 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 8 Å / σ(F): 2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |