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- PDB-1szy: Solution structure of ITALY1 ("Initiator tRNA Anticodon Loop from... -
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Basic information
Entry | Database: PDB / ID: 1szy | ||||||||||||||||||
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Title | Solution structure of ITALY1 ("Initiator tRNA Anticodon Loop from Yeast"), an unmodified 21-nt RNA with the sequence of the anticodon stem-loop of yeast initiator tRNA | ||||||||||||||||||
![]() | 5'-R(P*![]() RNA / Initiator tRNA Anticodon Loop | Function / homology | RNA / RNA (> 10) | ![]() Method | SOLUTION NMR / Distance geometry, simulated annealing | ![]() Schweisguth, D.C. / Moore, P.B. | ![]() ![]() Title: On the conformation of the anticodon loops of initiator and elongator methionine tRNAs. Authors: Schweisguth, D.C. / Moore, P.B. History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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-Validation report
Summary document | ![]() | 292 KB | Display | ![]() |
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Full document | ![]() | 291.9 KB | Display | |
Data in XML | ![]() | 1.5 KB | Display | |
Data in CIF | ![]() | 1.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: RNA chain | Mass: 6703.052 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: See DCS dissertation p. 61. |
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Sample preparation
Details | Contents: S1 NMR buffer; 100mM KCl, 50mM NaCl, 4mM Na cacodylate, 0.2mM EDTA |
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Sample conditions | pH: 6.5 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | ||||||||||||||||||||||||||||||
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Radiation wavelength | Relative weight: 1 | ||||||||||||||||||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: Distance geometry, simulated annealing / Software ordinal: 1 Details: RNA topology and parameters from Rife and Moore, NAR, 1996. Constraints in loop included 128 NOEs, 7 chi dihedrals inferred from NOEs, 20 v0-v4 ribose dihedrals, 8 a and z P dihedrals, 12 b ...Details: RNA topology and parameters from Rife and Moore, NAR, 1996. Constraints in loop included 128 NOEs, 7 chi dihedrals inferred from NOEs, 20 v0-v4 ribose dihedrals, 8 a and z P dihedrals, 12 b and g P dihedrals and 7 e P dihedrals. Stem was constrained to A-form based on qualitative spectral analysis. | ||||||||||||
NMR ensemble | Conformers submitted total number: 1 |