[English] 日本語
Yorodumi
- PDB-1szy: Solution structure of ITALY1 ("Initiator tRNA Anticodon Loop from... -

+
Open data


ID or keywords:

Loading...

-
Basic information

Entry
Database: PDB / ID: 1szy
TitleSolution structure of ITALY1 ("Initiator tRNA Anticodon Loop from Yeast"), an unmodified 21-nt RNA with the sequence of the anticodon stem-loop of yeast initiator tRNA
Components5'-R(P*GP*GP*CP*AP*GP*GP*GP*CP*UP*CP*AP*UP*AP*AP*CP*CP*CP*UP*GP*CP*C)-3'
KeywordsRNA / Initiator tRNA Anticodon Loop
Function / homologyRNA / RNA (> 10)
Function and homology information
MethodSOLUTION NMR / Distance geometry, simulated annealing
AuthorsSchweisguth, D.C. / Moore, P.B.
CitationJournal: J.Mol.Biol. / Year: 1997
Title: On the conformation of the anticodon loops of initiator and elongator methionine tRNAs.
Authors: Schweisguth, D.C. / Moore, P.B.
History
DepositionApr 6, 2004Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Apr 20, 2004Provider: repository / Type: Initial release
Revision 1.1Apr 30, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 2, 2022Group: Data collection / Database references / Derived calculations
Category: database_2 / pdbx_nmr_software ...database_2 / pdbx_nmr_software / pdbx_struct_assembly / pdbx_struct_oper_list
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name
Revision 1.4May 29, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

-
Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

-
Assembly

Deposited unit
A: 5'-R(P*GP*GP*CP*AP*GP*GP*GP*CP*UP*CP*AP*UP*AP*AP*CP*CP*CP*UP*GP*CP*C)-3'


Theoretical massNumber of molelcules
Total (without water)6,7031
Polymers6,7031
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

-
Components

#1: RNA chain 5'-R(P*GP*GP*CP*AP*GP*GP*GP*CP*UP*CP*AP*UP*AP*AP*CP*CP*CP*UP*GP*CP*C)-3'


Mass: 6703.052 Da / Num. of mol.: 1 / Source method: obtained synthetically

-
Experimental details

-
Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D NOESY
121DQF-COSY
13113C-1H HSQC
141TOCSY
151GE JRSE NOESY
16131P-1H COSY
17131P-1H heteroTOCSY
18131P-1H heteroTOCSY-NOESY
NMR detailsText: See DCS dissertation p. 61.

-
Sample preparation

DetailsContents: S1 NMR buffer; 100mM KCl, 50mM NaCl, 4mM Na cacodylate, 0.2mM EDTA
Sample conditionspH: 6.5 / Pressure: ambient / Temperature: 303 K

-
NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M
Radiation wavelengthRelative weight: 1
NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Yale homebuiltYalehomebuilt4901
Bruker AMBrukerAM5002
GE OMEGAGEOMEGA5003
Varian UNITYVarianUNITY5004
Varian UNITYPLUSVarianUNITYPLUS6005

-
Processing

NMR software
NameVersionClassification
Felixunknownprocessing
X-PLOR3.1structure solution
X-PLOR3.1refinement
RefinementMethod: Distance geometry, simulated annealing / Software ordinal: 1
Details: RNA topology and parameters from Rife and Moore, NAR, 1996. Constraints in loop included 128 NOEs, 7 chi dihedrals inferred from NOEs, 20 v0-v4 ribose dihedrals, 8 a and z P dihedrals, 12 b ...Details: RNA topology and parameters from Rife and Moore, NAR, 1996. Constraints in loop included 128 NOEs, 7 chi dihedrals inferred from NOEs, 20 v0-v4 ribose dihedrals, 8 a and z P dihedrals, 12 b and g P dihedrals and 7 e P dihedrals. Stem was constrained to A-form based on qualitative spectral analysis.
NMR ensembleConformers submitted total number: 1

+
About Yorodumi

-
News

-
Feb 9, 2022. New format data for meta-information of EMDB entries

New format data for meta-information of EMDB entries

  • Version 3 of the EMDB header file is now the official format.
  • The previous official version 1.9 will be removed from the archive.

Related info.:EMDB header

External links:wwPDB to switch to version 3 of the EMDB data model

-
Aug 12, 2020. Covid-19 info

Covid-19 info

URL: https://pdbj.org/emnavi/covid19.php

New page: Covid-19 featured information page in EM Navigator.

Related info.:Covid-19 info / Mar 5, 2020. Novel coronavirus structure data

+
Mar 5, 2020. Novel coronavirus structure data

Novel coronavirus structure data

Related info.:Yorodumi Speices / Aug 12, 2020. Covid-19 info

External links:COVID-19 featured content - PDBj / Molecule of the Month (242)：Coronavirus Proteases

+
Jan 31, 2019. EMDB accession codes are about to change! (news from PDBe EMDB page)

EMDB accession codes are about to change! (news from PDBe EMDB page)

  • The allocation of 4 digits for EMDB accession codes will soon come to an end. Whilst these codes will remain in use, new EMDB accession codes will include an additional digit and will expand incrementally as the available range of codes is exhausted. The current 4-digit format prefixed with “EMD-” (i.e. EMD-XXXX) will advance to a 5-digit format (i.e. EMD-XXXXX), and so on. It is currently estimated that the 4-digit codes will be depleted around Spring 2019, at which point the 5-digit format will come into force.
  • The EM Navigator/Yorodumi systems omit the EMD- prefix.

Related info.:Q: What is EMD? / ID/Accession-code notation in Yorodumi/EM Navigator

External links:EMDB Accession Codes are Changing Soon! / Contact to PDBj

+
Jul 12, 2017. Major update of PDB

Major update of PDB

  • wwPDB released updated PDB data conforming to the new PDBx/mmCIF dictionary.
  • This is a major update changing the version number from 4 to 5, and with Remediation, in which all the entries are updated.
  • In this update, many items about electron microscopy experimental information are reorganized (e.g. em_software).
  • Now, EM Navigator and Yorodumi are based on the updated data.

External links:wwPDB Remediation / Enriched Model Files Conforming to OneDep Data Standards Now Available in the PDB FTP Archive

-
Yorodumi

Thousand views of thousand structures

  • Yorodumi is a browser for structure data from EMDB, PDB, SASBDB, etc.
  • This page is also the successor to EM Navigator detail page, and also detail information page/front-end page for Omokage search.
  • The word "yorodu" (or yorozu) is an old Japanese word meaning "ten thousand". "mi" (miru) is to see.

Related info.:EMDB / PDB / SASBDB / Comparison of 3 databanks / Yorodumi Search / Aug 31, 2016. New EM Navigator & Yorodumi / Yorodumi Papers / Jmol/JSmol / Function and homology information / Changes in new EM Navigator and Yorodumi

Read more