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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1stc | ||||||
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タイトル | CAMP-DEPENDENT PROTEIN KINASE, ALPHA-CATALYTIC SUBUNIT IN COMPLEX WITH STAUROSPORINE | ||||||
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![]() | COMPLEX (TRANSFERASE/INHIBITOR) / PROTEIN KINASE / STAUROSPORINE / CAMP / PHOSPHORYLATION / COMPLEX (TRANSFERASE-INHIBITOR) / SERINE/THREONINE-PROTEIN KINASE / COMPLEX (TRANSFERASE-INHIBITOR) complex | ||||||
機能・相同性 | ![]() CD209 (DC-SIGN) signaling / HDL assembly / Regulation of insulin secretion / Rap1 signalling / Ion homeostasis / PKA activation in glucagon signalling / DARPP-32 events / CREB1 phosphorylation through the activation of Adenylate Cyclase / GPER1 signaling / Factors involved in megakaryocyte development and platelet production ...CD209 (DC-SIGN) signaling / HDL assembly / Regulation of insulin secretion / Rap1 signalling / Ion homeostasis / PKA activation in glucagon signalling / DARPP-32 events / CREB1 phosphorylation through the activation of Adenylate Cyclase / GPER1 signaling / Factors involved in megakaryocyte development and platelet production / Loss of Nlp from mitotic centrosomes / Recruitment of mitotic centrosome proteins and complexes / Loss of proteins required for interphase microtubule organization from the centrosome / Anchoring of the basal body to the plasma membrane / AURKA Activation by TPX2 / RET signaling / Interleukin-3, Interleukin-5 and GM-CSF signaling / Recruitment of NuMA to mitotic centrosomes / VEGFA-VEGFR2 Pathway / PKA activation / negative regulation of cAMP-dependent protein kinase activity / GLI3 is processed to GLI3R by the proteasome / MAPK6/MAPK4 signaling / Regulation of PLK1 Activity at G2/M Transition / Hedgehog 'off' state / cAMP-dependent protein kinase inhibitor activity / cAMP-dependent protein kinase / cAMP-dependent protein kinase activity / cAMP-dependent protein kinase complex / AMP-activated protein kinase activity / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / negative regulation of protein import into nucleus / Vasopressin regulates renal water homeostasis via Aquaporins / molecular function inhibitor activity / Mitochondrial protein degradation / protein kinase A regulatory subunit binding / protein kinase A catalytic subunit binding / mesoderm formation / sperm flagellum / negative regulation of TORC1 signaling / protein kinase A signaling / regulation of G2/M transition of mitotic cell cycle / acrosomal vesicle / neuromuscular junction / cellular response to heat / peptidyl-serine phosphorylation / molecular adaptor activity / protein kinase activity / protein domain specific binding / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / perinuclear region of cytoplasm / negative regulation of transcription by RNA polymerase II / mitochondrion / ATP binding / nucleus / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() ![]() | ||||||
![]() | Prade, L. / Engh, R.A. / Girod, A. / Kinzel, V. / Huber, R. / Bossemeyer, D. | ||||||
![]() | ![]() タイトル: Staurosporine-induced conformational changes of cAMP-dependent protein kinase catalytic subunit explain inhibitory potential. 著者: Prade, L. / Engh, R.A. / Girod, A. / Kinzel, V. / Huber, R. / Bossemeyer, D. #1: ![]() タイトル: Crystal Structures of Catalytic Subunit of Camp-Dependent Protein Kinase in Complex with Isoquinolinesulfonyl Protein Kinase Inhibitors H7, H8, and H89. Structural Implications for Selectivity 著者: Engh, R.A. / Girod, A. / Kinzel, V. / Huber, R. / Bossemeyer, D. #2: ![]() タイトル: Phosphotransferase and Substrate Binding Mechanism of the Camp-Dependent Protein Kinase Catalytic Subunit from Porcine Heart as Deduced from the 2.0 A Structure of the Complex with Mn2+ ...タイトル: Phosphotransferase and Substrate Binding Mechanism of the Camp-Dependent Protein Kinase Catalytic Subunit from Porcine Heart as Deduced from the 2.0 A Structure of the Complex with Mn2+ Adenylyl Imidodiphosphate and Inhibitor Peptide Pki(5-24) 著者: Bossemeyer, D. / Engh, R.A. / Kinzel, V. / Ponstingl, H. / Huber, R. #3: ![]() タイトル: A New Alkaloid Am-2282 of Streptomyces Origin. Taxonomy, Fermentation, Isolation and Preliminary Characterization 著者: Omura, S. / Iwai, Y. / Hirano, A. / Nakagawa, A. / Awaya, J. / Tsuchya, H. / Takahashi, Y. / Masuma, R. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 85.1 KB | 表示 | ![]() |
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PDB形式 | ![]() | 66.9 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 483.3 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 505.5 KB | 表示 | |
XML形式データ | ![]() | 11.8 KB | 表示 | |
CIF形式データ | ![]() | 17.5 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 1cdkS S: 精密化の開始モデル |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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要素
#1: タンパク質 | 分子量: 40786.395 Da / 分子数: 1 / 断片: CATALYTIC SUBUNIT / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() ![]() |
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#2: タンパク質・ペプチド | 分子量: 2226.411 Da / 分子数: 1 / 断片: INHIBITORY DOMAIN / 由来タイプ: 組換発現 / 参照: UniProt: P04541, UniProt: P61926*PLUS |
#3: 化合物 | ChemComp-STU / |
#4: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.24 Å3/Da / 溶媒含有率: 45.01 % | |||||||||||||||||||||||||
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結晶化 | *PLUS 温度: 5 ℃ / pH: 6.4 / 手法: 蒸気拡散法, ハンギングドロップ法 | |||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
回折 | 平均測定温度: 113 K |
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放射光源 | 由来: ![]() |
検出器 | タイプ: MARRESEARCH / 検出器: IMAGE PLATE / 日付: 1997年1月1日 |
放射 | モノクロメーター: GRAPHITE(002) / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.5418 Å / 相対比: 1 |
反射 | 解像度: 2.3→32 Å / Num. obs: 16993 / % possible obs: 92.2 % / 冗長度: 3.8 % / Rsym value: 0.073 |
反射 シェル | 解像度: 2.3→2.38 Å / Mean I/σ(I) obs: 3 / % possible all: 92.3 |
反射 | *PLUS Num. measured all: 64256 / Rmerge(I) obs: 0.073 |
反射 シェル | *PLUS % possible obs: 92.3 % / Rmerge(I) obs: 0.246 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 1CDK 解像度: 2.3→8 Å / σ(F): 2
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原子変位パラメータ | Biso mean: 34.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.3→8 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 2.3→2.38 Å / % reflection obs: 92.3 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ソフトウェア | *PLUS 名称: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Rfactor Rfree: 0.333 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS |