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- PDB-1srx: THREE-DIMENSIONAL STRUCTURE OF ESCHERICHIA COLI THIOREDOXIN-S2 TO... -

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Entry
Database: PDB / ID: 1srx
TitleTHREE-DIMENSIONAL STRUCTURE OF ESCHERICHIA COLI THIOREDOXIN-S2 TO 2.8 ANGSTROMS RESOLUTION
ComponentsTHIOREDOXIN
KeywordsELECTRON TRANSPORT
Function / homology
Function and homology information


DNA polymerase processivity factor activity / protein-disulfide reductase activity / cell redox homeostasis / cytoplasm / cytosol
Similarity search - Function
Thioredoxin / Thioredoxin / Thioredoxin, conserved site / Thioredoxin family active site. / Thioredoxin domain profile. / Thioredoxin domain / Thioredoxin-like superfamily
Similarity search - Domain/homology
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / Resolution: 2.8 Å
AuthorsSoderberg, B.-O.
Citation
Journal: Proc.Natl.Acad.Sci.USA / Year: 1975
Title: Three-dimensional structure of Escherichia coli thioredoxin-S2 to 2.8 A resolution.
Authors: Holmgren, A. / Soderberg, B.O. / Eklund, H. / Branden, C.I.
History
DepositionMay 6, 1976Processing site: BNL
Revision 1.0May 19, 1976Provider: repository / Type: Initial release
Revision 1.1Mar 3, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Source and taxonomy / Version format compliance
Revision 1.3Nov 29, 2017Group: Derived calculations / Other
Category: pdbx_database_status / struct_conf / struct_conf_type
Item: _pdbx_database_status.process_site
Revision 1.4Feb 14, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: THIOREDOXIN


Theoretical massNumber of molelcules
Total (without water)11,7591
Polymers11,7591
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)89.700, 51.100, 60.300
Angle α, β, γ (deg.)90.00, 113.50, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein THIOREDOXIN / Coordinate model: Cα atoms only


Mass: 11759.448 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Strain: B / References: UniProt: P0AA25
Compound detailsTHE ACTIVE SITE DISULFIDE BOND IS BETWEEN CYS-32 AND CYS-35 SITE S-S IS THE ACTIVE CENTER DISULFIDE ...THE ACTIVE SITE DISULFIDE BOND IS BETWEEN CYS-32 AND CYS-35 SITE S-S IS THE ACTIVE CENTER DISULFIDE BRIDGED REVERSE TURN. RESIDUES OTHER THAN THOSE GIVEN UNDER SITE BELOW MAY BE INVOLVED IN THE ACTIVITY.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 5.39 Å3/Da / Density % sol: 77.17 %
Crystal grow
*PLUS
Method: other / Details: Soderberg, B.O., (1974) J. Mol. Biol., 90, 143.

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Processing

RefinementHighest resolution: 2.8 Å
Refinement stepCycle: LAST / Highest resolution: 2.8 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms108 0 0 0 108

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