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Yorodumi- PDB-1sr3: Solution structure of the heme chaperone CcmE of Escherichia coli -
+Open data
-Basic information
Entry | Database: PDB / ID: 1sr3 | |||||||||
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Title | Solution structure of the heme chaperone CcmE of Escherichia coli | |||||||||
Components | APO-CCME | |||||||||
Keywords | CHAPERONE / OB fold / beta barrel / flexible C-terminal domain | |||||||||
Function / homology | Function and homology information cytochrome c biosynthetic process / heme import across plasma membrane / cytochrome complex assembly / ATP-binding cassette (ABC) transporter complex / heme binding / metal ion binding Similarity search - Function | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | SOLUTION NMR | |||||||||
Authors | Enggist, E. / Thony-Meyer, L. / Guntert, P. / Pervushin, K. | |||||||||
Citation | Journal: Structure / Year: 2002 Title: NMR Structure of the Heme Chaperone Ccme Reveals a Novel Functional Motif Authors: Enggist, E. / Thony-Meyer, L. / Guntert, P. / Pervushin, K. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sr3.cif.gz | 693.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sr3.ent.gz | 598.2 KB | Display | PDB format |
PDBx/mmJSON format | 1sr3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sr/1sr3 ftp://data.pdbj.org/pub/pdb/validation_reports/sr/1sr3 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 15349.208 Da / Num. of mol.: 1 / Fragment: SOLUBLE APO-CCME-H6(C-TERMINAL DOMAIN) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Cellular location: PERIPLASM / Gene: CCME / Plasmid: PEC415 / Production host: Escherichia coli (E. coli) / Strain (production host): EC06 / References: UniProt: P69490 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.1MM APO-CCME-H6 U-98% 13C; U-98% 15N; 50MM PHOSPHATE BUFFER, PH 7.2, 300MM NACL, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | pH: 7.2 / Pressure: AMBIENT / Temperature: 303 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |