Journal: Biochemistry / Year: 1995 Title: Binding to protein targets of peptidic leads discovered by phage display: crystal structures of streptavidin-bound linear and cyclic peptide ligands containing the HPQ sequence Authors: Katz, B.A.
MTRIX THE TRANSFORMATIONS PRESENTED ON MTRIX RECORDS BELOW DESCRIBE NON-CRYSTALLOGRAPHIC RELATIONSHIPS AMONG THE VARIOUS DOMAINS IN THIS ENTRY. APPLYING THE APPROPRIATE MTRIX TRANSFORMATION TO THE RESIDUES LISTED FIRST WILL YIELD APPROXIMATE COORDINATES FOR THE RESIDUES LISTED SECOND. NONCRYSTALLOGRAPHIC TWO-FOLD RELATING PROTOMERS OF THE STREPTAVIDIN TETRAMER APPLIED TO TRANSFORMED TO MTRIX RESIDUES RESIDUES RMSD M1 B 13 .. M 7 D 13 .. P 7 0.845 SYMMETRY THE CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS PRESENTED BELOW GENERATE THE SUBUNITS OF THE POLYMERIC MOLECULE. STREPTAVIDIN IS A TETRAMERIC PROTEIN. THE CRYSTALLOGRAPHIC TRANSFORMATION GIVEN HERE GENERATES THE TETRAMER FROM THE DIMER FOUND IN THE ASYMMETRIC UNIT OF THE CRYSTALS. APPLIED TO RESIDUES: B 13 .. B 133 D 13 .. D 133 M 2 .. M 6 P 1 .. P 6 SYMMETRY1 1 1.000000 0.000000 0.000000 0.00000 SYMMETRY2 1 0.000000 -1.000000 0.000000 0.00000 SYMMETRY3 1 0.000000 0.000000 -1.000000 0.00000
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Components
#1: Protein
STREPTAVIDIN
Mass: 14181.324 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Streptomyces avidinii (bacteria) / References: UniProt: P22629
#2: Protein/peptide
FCHPQNT
Mass: 830.864 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Highest resolution: 1.74 Å / Lowest resolution: 50 Å / Num. measured all: 94347 / Rmerge(I) obs: 0.072
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Processing
Software
Name
Version
Classification
X-PLOR
modelbuilding
X-PLOR
refinement
SADIE
datareduction
SAINT
(SIEMENS)
datareduction
X-PLOR
phasing
Refinement
Resolution: 1.76→7.5 Å / σ(F): 1 Details: CRYST1 CELL AXES CHOSEN TO CORRESPOND TO COORDINATES OF STREPTAVIDIN DEPOSITED BY WEBER ET AL. IN THE PDB (ENTRY 1PTS). THE FOLLOWING ATOMS HAD WEAK DENSITY AND OCCUPANCIES WERE REFINED: B ...Details: CRYST1 CELL AXES CHOSEN TO CORRESPOND TO COORDINATES OF STREPTAVIDIN DEPOSITED BY WEBER ET AL. IN THE PDB (ENTRY 1PTS). THE FOLLOWING ATOMS HAD WEAK DENSITY AND OCCUPANCIES WERE REFINED: B 13, B 14, B 15 AND (NOT NAME C OR NAME O) D 13, D 14, D 15 AND (NOT NAME C OR NAME O) D 46, D 47, D 48, D 49, D 50, D 51 AND (NOT NAME C OR NAME O) B 46, B 47, B 48, B 49, B 50 AND (NOT NAME C OR NAME O) B 67, B 68 AND (NOT NAME C OR NAME O) B 53 AND (NAME NE OR NAME NH1 OR NAME NH2 OR NAME CZ) B 103 AND (NAME NE OR NAME NH1 OR NAME NH2 OR NAME CZ) D 103 AND (NAME NE OR NAME NH1 OR NAME NH2 OR NAME CZ) D 84 AND (NAME NE OR NAME NH1 OR NAME NH2 OR NAME CZ) B 116 AND (NAME CG OR NAME OR NAME OE1 OR NAME OE2) M 7, P 1, P 2, B 135 M 1 WAS NOT LOCATED OR INCLUDED IN THE MODEL. DISCRETELY DISORDERED SIDE CHAINS WHOSE OCCUPANCIES AND STRUCTURES WERE SIMULTANEOUSLY REFINED WERE B 73, D 73, B 110, D 110, B 22, D 107, D 105. B 22 IS DISORDERED BETWEEN 2 CONFORMATIONS ONE OF WHICH OCCUPIES A SIMILAR REGION OF SPACE AS A TWO-FOLD RELATED B 22. THIS DISORDER CAN NOT BE PROPERLY REFINED WITH X-PLOR. SEVERAL WATERS ARE ON OR NEAR TWO-FOLD AXES, AND CAN NOT BE PROPERLY REFINED WITH X-PLOR. BULK SOLVENT WAS REFINED.
Rfactor
Num. reflection
Rfree
0.222
-
Rwork
0.187
-
obs
0.187
20506
Displacement parameters
Biso mean: 22 Å2
Refinement step
Cycle: LAST / Resolution: 1.76→7.5 Å
Protein
Nucleic acid
Ligand
Solvent
Total
Num. atoms
2446
0
0
477
2923
Refine LS restraints
Refine-ID
Type
Dev ideal
X-RAY DIFFRACTION
x_bond_d
0.019
X-RAY DIFFRACTION
x_bond_d_na
X-RAY DIFFRACTION
x_bond_d_prot
X-RAY DIFFRACTION
x_angle_d
X-RAY DIFFRACTION
x_angle_d_na
X-RAY DIFFRACTION
x_angle_d_prot
X-RAY DIFFRACTION
x_angle_deg
3.4
X-RAY DIFFRACTION
x_angle_deg_na
X-RAY DIFFRACTION
x_angle_deg_prot
X-RAY DIFFRACTION
x_dihedral_angle_d
27.7
X-RAY DIFFRACTION
x_dihedral_angle_d_na
X-RAY DIFFRACTION
x_dihedral_angle_d_prot
X-RAY DIFFRACTION
x_improper_angle_d
X-RAY DIFFRACTION
x_improper_angle_d_na
X-RAY DIFFRACTION
x_improper_angle_d_prot
X-RAY DIFFRACTION
x_mcbond_it
X-RAY DIFFRACTION
x_mcangle_it
X-RAY DIFFRACTION
x_scbond_it
X-RAY DIFFRACTION
x_scangle_it
Software
*PLUS
Name: X-PLOR / Classification: refinement
Refinement
*PLUS
Solvent computation
*PLUS
Displacement parameters
*PLUS
Refine LS restraints
*PLUS
Refine-ID
Type
Dev ideal
X-RAY DIFFRACTION
x_dihedral_angle_d
X-RAY DIFFRACTION
x_dihedral_angle_deg
27.7
+
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