+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1sha | ||||||
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タイトル | CRYSTAL STRUCTURE OF THE PHOSPHOTYROSINE RECOGNITION DOMAIN SH2 OF V-SRC COMPLEXED WITH TYROSINE-PHOSPHORYLATED PEPTIDES | ||||||
要素 |
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キーワード | PHOSPHOTRANSFERASE | ||||||
機能・相同性 | 機能・相同性情報 platelet activating factor receptor activity / platelet-derived growth factor receptor activity / platelet-derived growth factor beta-receptor activity / cell migration involved in coronary angiogenesis / metanephric glomerular mesangial cell proliferation involved in metanephros development / positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway / smooth muscle cell chemotaxis / metanephric glomerular capillary formation / cell migration involved in vasculogenesis / aorta morphogenesis ...platelet activating factor receptor activity / platelet-derived growth factor receptor activity / platelet-derived growth factor beta-receptor activity / cell migration involved in coronary angiogenesis / metanephric glomerular mesangial cell proliferation involved in metanephros development / positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway / smooth muscle cell chemotaxis / metanephric glomerular capillary formation / cell migration involved in vasculogenesis / aorta morphogenesis / positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway / platelet-derived growth factor binding / vascular endothelial growth factor binding / retina vasculature development in camera-type eye / cardiac myofibril assembly / phosphatidylinositol metabolic process / positive regulation of chemotaxis / Signaling by PDGF / platelet-derived growth factor receptor binding / osteoclast development / positive regulation of DNA biosynthetic process / positive regulation of smooth muscle cell migration / positive regulation of calcium ion import / platelet-derived growth factor receptor-beta signaling pathway / progesterone receptor signaling pathway / positive regulation of phosphoprotein phosphatase activity / platelet-derived growth factor receptor signaling pathway / : / positive regulation of calcium-mediated signaling / negative regulation of intrinsic apoptotic signaling pathway / bone resorption / extrinsic component of cytoplasmic side of plasma membrane / lysosomal lumen / cell chemotaxis / Downstream signal transduction / positive regulation of mitotic nuclear division / regulation of actin cytoskeleton organization / negative regulation of extrinsic apoptotic signaling pathway / positive regulation of smooth muscle cell proliferation / non-specific protein-tyrosine kinase / positive regulation of MAP kinase activity / non-membrane spanning protein tyrosine kinase activity / epidermal growth factor receptor signaling pathway / 受容体型チロシンキナーゼ / peptidyl-tyrosine phosphorylation / Constitutive Signaling by Aberrant PI3K in Cancer / positive regulation of reactive oxygen species metabolic process / PIP3 activates AKT signaling / PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling / cytoplasmic vesicle / RAF/MAP kinase cascade / protein tyrosine kinase activity / protein autophosphorylation / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / positive regulation of ERK1 and ERK2 cascade / receptor complex / 細胞接着 / protein kinase activity / positive regulation of cell migration / apical plasma membrane / リン酸化 / signaling receptor binding / intracellular membrane-bounded organelle / focal adhesion / 自然免疫系 / positive regulation of cell population proliferation / protein kinase binding / ゴルジ体 / enzyme binding / シグナル伝達 / ATP binding / 生体膜 / 細胞核 / 細胞膜 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Rous sarcoma virus (ラウス肉腫ウイルス) | ||||||
手法 | X線回折 / 解像度: 1.5 Å | ||||||
データ登録者 | Waksman, G. / Kuriyan, J. | ||||||
引用 | ジャーナル: Nature / 年: 1992 タイトル: Crystal structure of the phosphotyrosine recognition domain SH2 of v-src complexed with tyrosine-phosphorylated peptides. 著者: Waksman, G. / Kominos, D. / Robertson, S.C. / Pant, N. / Baltimore, D. / Birge, R.B. / Cowburn, D. / Hanafusa, H. / Mayer, B.J. / Overduin, M. / Resh, M.D. / Rios, C.B. / Silverman, L. / Kuriyan, J. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1sha.cif.gz | 34.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1sha.ent.gz | 23.7 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1sha.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/sh/1sha ftp://data.pdbj.org/pub/pdb/validation_reports/sh/1sha | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 11970.480 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) Rous sarcoma virus (ラウス肉腫ウイルス) 属: Alpharetrovirusアルファレトロウイルス属 / 参照: UniProt: P00524, EC: 2.7.1.112 |
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#2: タンパク質・ペプチド | 分子量: 701.767 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) Rous sarcoma virus (ラウス肉腫ウイルス) 属: Alpharetrovirusアルファレトロウイルス属 / 参照: UniProt: P09619*PLUS |
#3: 水 | ChemComp-HOH / |
-実験情報
-実験
実験 | 手法: X線回折 |
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-試料調製
結晶 | マシュー密度: 2.08 Å3/Da / 溶媒含有率: 40.77 % | ||||||||||||||||||||
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結晶化 | *PLUS pH: 8 / 手法: 蒸気拡散法 | ||||||||||||||||||||
溶液の組成 | *PLUS
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-解析
ソフトウェア |
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精密化 | 解像度: 1.5→6 Å / Rfactor Rwork: 0.208 / Rfactor obs: 0.208 / σ(F): 2 詳細: THE LOOP FROM RESIDUE 49 TO 53 IS DISORDERED AND IS NOT CLEARLY VISIBLE IN THE ELECTRON DENSITY. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 1.5→6 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: X-PLOR / 分類: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Rfactor obs: 0.208 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS タイプ: x_angle_d / Dev ideal: 2.9 |