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Open data
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Basic information
Entry | Database: PDB / ID: 1sei | ||||||
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Title | STRUCTURE OF 30S RIBOSOMAL PROTEIN S8 | ||||||
![]() | RIBOSOMAL PROTEIN S8 | ||||||
![]() | RIBOSOMAL PROTEIN / PROKARYOTIC / RRNA-BINDING | ||||||
Function / homology | ![]() rRNA binding / ribosome / structural constituent of ribosome / translation Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Davies, C. / Ramakrishnan, V. / White, S.W. | ||||||
![]() | ![]() Title: Structural evidence for specific S8-RNA and S8-protein interactions within the 30S ribosomal subunit: ribosomal protein S8 from Bacillus stearothermophilus at 1.9 A resolution. Authors: Davies, C. / Ramakrishnan, V. / White, S.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 62 KB | Display | ![]() |
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PDB format | ![]() | 47.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 369.5 KB | Display | ![]() |
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Full document | ![]() | 374.5 KB | Display | |
Data in XML | ![]() | 6.6 KB | Display | |
Data in CIF | ![]() | 10.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.9987, 0.04913, 0.01345), Vector: |
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Components
#1: Protein | Mass: 14653.166 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Description: T7 EXPRESSION SYSTEM INDUCIBLE BY ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE (IPTG). ALSO SEE MEDLINE IDENTIFIER 92110431. Gene: BACILLUS STEAROTHERMOPHILUS / Plasmid: PET13 / Species (production host): Escherichia coli / Gene (production host): BACILLUS STEAROTHERMOPHILUS / Production host: ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.3 % |
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Crystal grow | *PLUS Temperature: 22 ℃ / Method: vapor diffusion, hanging drop |
Components of the solutions | *PLUS Common name: sodium chloride |
-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jun 7, 1996 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Num. obs: 21973 / % possible obs: 94.2 % / Observed criterion σ(I): 0 / Redundancy: 5.05 % / Rmerge(I) obs: 0.083 |
Reflection | *PLUS Highest resolution: 1.9 Å |
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Processing
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Refinement | Resolution: 1.9→8 Å / σ(F): 0
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Displacement parameters | Biso mean: 27.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 2 Å / % reflection Rfree: 0.35 % / Total num. of bins used: 8 / Rfactor obs: 0.31 |