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Yorodumi- PDB-1sat: CRYSTAL STRUCTURE OF THE 50 KDA METALLO PROTEASE FROM S. MARCESCENS -
+Open data
-Basic information
Entry | Database: PDB / ID: 1sat | ||||||
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Title | CRYSTAL STRUCTURE OF THE 50 KDA METALLO PROTEASE FROM S. MARCESCENS | ||||||
Components | SERRATIA PROTEASE | ||||||
Keywords | HYDROLASE (SERINE PROTEASE) / PARALLEL BETA HELIX / PARALLEL BETA ROLL | ||||||
Function / homology | Function and homology information serralysin / symbiont-mediated killing of host cell / extracellular matrix / metalloendopeptidase activity / toxin activity / calcium ion binding / proteolysis / extracellular space / zinc ion binding / extracellular region Similarity search - Function | ||||||
Biological species | Serratia marcescens (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.75 Å | ||||||
Authors | Baumann, U. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1994 Title: Crystal structure of the 50 kDa metallo protease from Serratia marcescens. Authors: Baumann, U. #1: Journal: Embo J. / Year: 1993 Title: Three-Dimensional Structure of the Alkaline Protease from Ps. Aeruginosa: A Two-Domain Protein with a Calcium Binding Parallel Beta Roll Motif Authors: Baumann, U. / Wu, S. / Flaherty, K.M. / Mckay, D.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sat.cif.gz | 105.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sat.ent.gz | 79.8 KB | Display | PDB format |
PDBx/mmJSON format | 1sat.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sa/1sat ftp://data.pdbj.org/pub/pdb/validation_reports/sa/1sat | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 50313.082 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Serratia marcescens (bacteria) / References: UniProt: P23694 | ||
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#2: Chemical | ChemComp-ZN / | ||
#3: Chemical | ChemComp-CA / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.49 Å3/Da / Density % sol: 64.75 % | ||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 4 / 20 ℃ / pH: 6.3 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | Num. obs: 81141 / % possible obs: 95 % / Observed criterion σ(I): 0 |
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Reflection | *PLUS Highest resolution: 1.7 Å / Num. measured all: 253857 / Rmerge(I) obs: 0.075 |
Reflection shell | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 1.75 Å / % possible obs: 82 % |
-Processing
Software |
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Refinement | Resolution: 1.75→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 19.44 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.75→8 Å
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Refine LS restraints |
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Refine LS restraints | *PLUS
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