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Open data
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Basic information
Entry | Database: PDB / ID: 1rsu | ||||||
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Title | COMPLEX BETWEEN STREPTAVIDIN AND THE STREP-TAG II PEPTIDE | ||||||
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![]() | COMPLEX (SIGNAL PROTEIN/PEPTIDE) / COMPLEX (SIGNAL PROTEIN-PEPTIDE) / ![]() | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Koepke, J. / Schmidt, T. / Skerra, A. | ||||||
![]() | ![]() Title: Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin. Authors: Schmidt, T.G. / Koepke, J. / Frank, R. / Skerra, A. #1: ![]() Title: One-Step Affinity Purification of Bacterially Produced Proteins by Means of the 'Strep Tag' and Immobilized Recombinant Core Streptavidin Authors: Schmidt, T.G. / Skerra, A. #2: ![]() Title: The Random Peptide Library-Assisted Engineering of a C-Terminal Affinity Peptide, Useful for the Detection and Purification of a Functional Ig Fv Fragment Authors: Schmidt, T.G. / Skerra, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 48.8 KB | Display | ![]() |
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PDB format | ![]() | 35.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | ![]() Mass: 13341.454 Da / Num. of mol.: 1 / Fragment: RESIDUES 13 - 139 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 1261.341 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.58 Å3/Da / Density % sol: 49 % | ||||||||||||||||||||||||
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Crystal grow![]() | *PLUS pH: 7 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 |
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Detector | Type: RIGAKU / Detector: IMAGE PLATE / Date: Jun 13, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.7→37.38 Å / Num. obs: 16909 / % possible obs: 97.2 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.0923 |
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Processing
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Refinement | Resolution: 1.7→8 Å / σ(F): 2
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Displacement parameters | Biso mean: 20.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.18 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→8 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor obs: 0.18 / Rfactor Rwork![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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