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- PDB-1rlz: Deoxyhypusine synthase holoenzyme in its high ionic strength, low... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1rlz | ||||||
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Title | Deoxyhypusine synthase holoenzyme in its high ionic strength, low pH crystal form | ||||||
![]() | Deoxyhypusine synthase | ||||||
![]() | TRANSFERASE / Rossmann Fold / NAD cofactor / deoxyhypusine / hypusine / spermidine | ||||||
Function / homology | ![]() deoxyhypusine synthase / Hypusine synthesis from eIF5A-lysine / peptidyl-lysine modification to peptidyl-hypusine / deoxyhypusine synthase activity / spermidine metabolic process / spermidine catabolic process / positive regulation of T cell proliferation / glucose homeostasis / translation / positive regulation of cell population proliferation ...deoxyhypusine synthase / Hypusine synthesis from eIF5A-lysine / peptidyl-lysine modification to peptidyl-hypusine / deoxyhypusine synthase activity / spermidine metabolic process / spermidine catabolic process / positive regulation of T cell proliferation / glucose homeostasis / translation / positive regulation of cell population proliferation / identical protein binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Umland, T.C. / Wolff, E.C. / Park, M.-H. / Davies, D.R. | ||||||
![]() | ![]() Title: A New Crystal Structure of Deoxyhypusine Synthase Reveals the Configuration of the Active Enzyme and of an Enzyme-NAD-Inhibitor Ternary Complex Authors: Umland, T.C. / Wolff, E.C. / Park, M.-H. / Davies, D.R. #1: ![]() Title: Crystal structure of the NAD complex of human deoxyhypusine synthase: an enzyme with a ball-and-chain mechanism for blocking the active site Authors: Liao, D.I. / Wolff, E.C. / Park, M.-H. / Davies, D.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 84.3 KB | Display | ![]() |
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PDB format | ![]() | 62.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1rozC ![]() 1rqdC ![]() 1dhsS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Details | The biological assembly is a tetramer generated from the monomer in the asymmetric unit by the crystallographic operations: -y, -x, 69.83-z; -x, -y, z; and y, x, 69.83-z |
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Components
#1: Protein | Mass: 41012.434 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-NAD / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 50.98 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.5 Details: 1.7M sodium/potassium phosphate, 100mM Hepes (pH 7.5), NAD, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Nov 30, 1998 / Details: mirrors |
Radiation | Monochromator: Ni filter / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→100 Å / Num. all: 24976 / Num. obs: 22384 / % possible obs: 89.5 % / Observed criterion σ(I): -3 / Redundancy: 4.66 % / Biso Wilson estimate: 12.4 Å2 / Rsym value: 0.058 / Net I/σ(I): 16.1 |
Reflection shell | Resolution: 2.1→2.15 Å / Redundancy: 2.35 % / Num. unique all: 1104 / Rsym value: 0.174 / % possible all: 67.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1DHS Resolution: 2.15→50 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 17.5 Å2 | |||||||||||||||||||||||||
Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.15→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.15→2.23 Å / Rfactor Rfree error: 0.007
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