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- PDB-1reg: CRYSTAL STRUCTURE OF THE T4 REGA TRANSLATIONAL REGULATOR PROTEIN ... -

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Basic information

Entry
Database: PDB / ID: 1reg
TitleCRYSTAL STRUCTURE OF THE T4 REGA TRANSLATIONAL REGULATOR PROTEIN AT 1.9 ANGSTROMS RESOLUTION
ComponentsT4 REGA
KeywordsTRANSLATIONAL REGULATOR PROTEIN
Function / homology
Function and homology information


regulation of translation / RNA binding
Similarity search - Function
Translation repressor RegA / Translation repressor RegA / Translation repressor RegA superfamily / Bacteriophage translational regulator / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Translation repressor protein
Similarity search - Component
Biological speciesEnterobacteria phage T4 (virus)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.9 Å
AuthorsKang, C. / Rich, A.
CitationJournal: Science / Year: 1995
Title: Crystal structure of the T4 regA translational regulator protein at 1.9 A resolution.
Authors: Kang, C. / Chan, R. / Berger, I. / Lockshin, C. / Green, L. / Gold, L. / Rich, A.
History
DepositionJan 11, 1995Processing site: BNL
Revision 1.0Jan 1, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 14, 2024Group: Data collection / Database references / Other
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
X: T4 REGA
Y: T4 REGA


Theoretical massNumber of molelcules
Total (without water)29,2862
Polymers29,2862
Non-polymers00
Water1,27971
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1300 Å2
ΔGint-7 kcal/mol
Surface area13400 Å2
MethodPISA
Unit cell
Length a, b, c (Å)82.708, 85.725, 43.485
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein T4 REGA


Mass: 14643.151 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Enterobacteria phage T4 (virus) / Genus: T4-like viruses / Species: Enterobacteria phage T4 sensu lato / References: UniProt: P69702
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 71 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.63 Å3/Da / Density % sol: 53.26 %
Crystal grow
*PLUS
pH: 8.5 / Method: unknown
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDChemical formulaDetails
13 mg/mlprotein 1drop
212.5 %(w/v)PEG40001drop
30.01 M1dropMgCl2
40.05 MTris-HCl1drop
625 %(w/v)PEG40001reservoir
5salt1reservoirtwice the concentration

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Data collection

Diffraction
IDCrystal-ID
11
21
Diffraction source
SourceSiteBeamlineIDWavelength (Å)
SYNCHROTRONNSLS X12C11.1
ROTATING ANODE21.5418
Detector
TypeIDDetectorDate
MARRESEARCH1IMAGE PLATEAug 20, 1994
RIGAKU RAXIS IIC2IMAGE PLATEMay 1, 1994
RadiationMonochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
IDWavelength (Å)Relative weight
11.11
21.54181
ReflectionNum. obs: 18037 / % possible obs: 74 % / Rmerge(I) obs: 0.045
Reflection
*PLUS
Highest resolution: 1.9 Å / Observed criterion σ(I): 2 / Rmerge(I) obs: 0.045

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Processing

Software
NameVersionClassification
X-PLOR3model building
X-PLOR3refinement
X-PLOR3phasing
RefinementResolution: 1.9→7 Å / σ(F): 2
RfactorNum. reflection
Rfree0.214 -
Rwork0.18 -
obs0.18 12508
Displacement parametersBiso mean: 16.53 Å2
Refinement stepCycle: LAST / Resolution: 1.9→7 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms2040 0 0 71 2111
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONx_bond_d0.015
X-RAY DIFFRACTIONx_bond_d_na
X-RAY DIFFRACTIONx_bond_d_prot
X-RAY DIFFRACTIONx_angle_d
X-RAY DIFFRACTIONx_angle_d_na
X-RAY DIFFRACTIONx_angle_d_prot
X-RAY DIFFRACTIONx_angle_deg3.25
X-RAY DIFFRACTIONx_angle_deg_na
X-RAY DIFFRACTIONx_angle_deg_prot
X-RAY DIFFRACTIONx_dihedral_angle_d
X-RAY DIFFRACTIONx_dihedral_angle_d_na
X-RAY DIFFRACTIONx_dihedral_angle_d_prot
X-RAY DIFFRACTIONx_improper_angle_d
X-RAY DIFFRACTIONx_improper_angle_d_na
X-RAY DIFFRACTIONx_improper_angle_d_prot
X-RAY DIFFRACTIONx_mcbond_it
X-RAY DIFFRACTIONx_mcangle_it
X-RAY DIFFRACTIONx_scbond_it
X-RAY DIFFRACTIONx_scangle_it
Refinement
*PLUS
Solvent computation
*PLUS
Displacement parameters
*PLUS

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