[English] 日本語
Yorodumi- PDB-1r8t: Solution structures of high affinity miniprotein ligands to Strep... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1r8t | ||||||
---|---|---|---|---|---|---|---|
Title | Solution structures of high affinity miniprotein ligands to Streptavidin | ||||||
Components | MP1 | ||||||
Keywords | UNKNOWN FUNCTION / miniprotein / streptavidin / high-affinity | ||||||
Method | SOLUTION NMR / distance geometry, simulated annealing | ||||||
Authors | Luo, J. / Mukherjee, M. / Fan, X. / Yang, H. / Liu, D. / Khan, R. / White, M. / Fox, R.O. | ||||||
Citation | Journal: To be Published Title: Structure-based design of high affinity miniprotein ligands Authors: Luo, J. / Mukherjee, M. / Fan, X. / Yang, H. / Liu, D. / Khan, R. / White, M. / Fox, R.O. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1r8t.cif.gz | 111.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1r8t.ent.gz | 88.3 KB | Display | PDB format |
PDBx/mmJSON format | 1r8t.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/r8/1r8t ftp://data.pdbj.org/pub/pdb/validation_reports/r8/1r8t | HTTPS FTP |
---|
-Related structure data
Related structure data | |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein/peptide | Mass: 1687.003 Da / Num. of mol.: 1 / Source method: obtained synthetically Details: This sequence was identified to be a high affinity streptavidin binding ligand by phage display |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|---|
NMR details | Text: This structure was determined using standard 2D homonuclear experiments. |
-Sample preparation
Details | Contents: 1.3 mM MP1, 20mM sodium acetate(d3), pH 5.3, 90% H2O and 10% D2O Solvent system: 90% H2O and 10% D2O |
---|---|
Sample conditions | pH: 5.3 / Pressure: ambient / Temperature: 283 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
---|---|
Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian UNITYPLUS / Manufacturer: Varian / Model: UNITYPLUS / Field strength: 750 MHz |
-Processing
NMR software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: distance geometry, simulated annealing / Software ordinal: 1 | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 30 |