+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1r7f | ||||||
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タイトル | NMR structure of the membrane anchor domain (1-31) of the nonstructural protein 5A (NS5A) of hepatitis C virus (Ensemble of 43 structures. Sample in 100mM SDS) | ||||||
要素 | Genome polyprotein | ||||||
キーワード | MEMBRANE PROTEIN / Membrane anchor domain / HCV NS5A protein / peptide. | ||||||
機能・相同性 | 機能・相同性情報 positive regulation of hexokinase activity / modulation by virus of host cellular process / translocation of peptides or proteins into host cell cytoplasm / Toll-like receptor 2 binding / viral capsid assembly / adhesion receptor-mediated virion attachment to host cell / TBC/RABGAPs / hepacivirin / host cell mitochondrial membrane / host cell lipid droplet ...positive regulation of hexokinase activity / modulation by virus of host cellular process / translocation of peptides or proteins into host cell cytoplasm / Toll-like receptor 2 binding / viral capsid assembly / adhesion receptor-mediated virion attachment to host cell / TBC/RABGAPs / hepacivirin / host cell mitochondrial membrane / host cell lipid droplet / symbiont-mediated suppression of host TRAF-mediated signal transduction / transformation of host cell by virus / positive regulation of cytokinesis / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / negative regulation of protein secretion / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / endoplasmic reticulum-Golgi intermediate compartment membrane / kinase binding / SH3 domain binding / nucleoside-triphosphate phosphatase / protein complex oligomerization / monoatomic ion channel activity / viral nucleocapsid / clathrin-dependent endocytosis of virus by host cell / entry receptor-mediated virion attachment to host cell / 加水分解酵素; プロテアーゼ; ペプチド結合加水分解酵素; システインプロテアーゼ / RNA helicase activity / host cell perinuclear region of cytoplasm / host cell endoplasmic reticulum membrane / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / RNA helicase / ribonucleoprotein complex / induction by virus of host autophagy / RNA-directed RNA polymerase / viral RNA genome replication / cysteine-type endopeptidase activity / serine-type endopeptidase activity / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / fusion of virus membrane with host endosome membrane / viral envelope / host cell nucleus / apoptotic process / host cell plasma membrane / structural molecule activity / virion membrane / negative regulation of transcription by RNA polymerase II / ATP hydrolysis activity / proteolysis / RNA binding / zinc ion binding / ATP binding 類似検索 - 分子機能 | ||||||
手法 | 溶液NMR / distance geometry, simulated annealing, molecular dynamics, energy minimization | ||||||
データ登録者 | Penin, F. / Brass, V. / Appel, N. / Ramboarina, S. / Montserret, R. / Ficheux, D. / Blum, H.E. / Bartenschlager, R. / Moradpour, D. | ||||||
引用 | ジャーナル: J.Biol.Chem. / 年: 2004 タイトル: Structure and function of the membrane anchor domain of hepatitis C virus nonstructural protein 5A. 著者: Penin, F. / Brass, V. / Appel, N. / Ramboarina, S. / Montserret, R. / Ficheux, D. / Blum, H.E. / Bartenschlager, R. / Moradpour, D. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1r7f.cif.gz | 441.6 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1r7f.ent.gz | 368.7 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1r7f.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/r7/1r7f ftp://data.pdbj.org/pub/pdb/validation_reports/r7/1r7f | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 3770.423 Da / 分子数: 1 断片: Nonstructural protein NS5A (P56)(residues 1973-2003 of Swiss-Prot sequence P27958) 由来タイプ: 合成 詳細: The peptide was chemically synthesized. The sequence is naturally found in hepatitis C virus. 参照: UniProt: P27958 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | 内容: 1.2mM NS5A[1-31], 10mM DTTd10 / 溶媒系: 100mM SDS in H2O/D2O 95/5 (v/v) |
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試料状態 | pH: 6 / 圧: ambient / 温度: 313 K |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Varian UNITYPLUS / 製造業者: Varian / モデル: UNITYPLUS / 磁場強度: 500 MHz |
-解析
NMR software |
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精密化 | 手法: distance geometry, simulated annealing, molecular dynamics, energy minimization ソフトェア番号: 1 | ||||||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the least restraint violations 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 43 |