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- PDB-1r23: Crystal structure of the cyanobacterial metallothionein repressor... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1r23 | ||||||
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Title | Crystal structure of the cyanobacterial metallothionein repressor SmtB in the Zn1-form (one Zn(II) per dimer) | ||||||
![]() | Transcriptional repressor smtB | ||||||
![]() | TRANSCRIPTION REPRESSOR / Zinc / DNA binding Transcriptional regulation / winged HTH protein | ||||||
Function / homology | ![]() DNA-binding transcription factor activity / DNA binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Eicken, C. / Pennella, M.A. / Chen, X. / Koshlap, K.M. / VanZile, M.L. / Sacchettini, J.C. / Giedroc, D.P. | ||||||
![]() | ![]() Title: A metal-ligand-mediated intersubunit allosteric switch in related SmtB/ArsR zinc sensor proteins. Authors: Eicken, C. / Pennella, M.A. / Chen, X. / Koshlap, K.M. / VanZile, M.L. / Sacchettini, J.C. / Giedroc, D.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 54.6 KB | Display | ![]() |
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PDB format | ![]() | 39.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 373.6 KB | Display | ![]() |
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Full document | ![]() | 375.6 KB | Display | |
Data in XML | ![]() | 5.4 KB | Display | |
Data in CIF | ![]() | 8.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1r1tSC ![]() 1r1uC ![]() 1r1vC ![]() 1r22C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The biological assembly is a homodimer as present in the asymmetric unit. |
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Components
#1: Protein | Mass: 13561.474 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Species: Synechococcus elongatus / Strain: PCC7942 / Gene: SMTB / Species (production host): Escherichia coli / Production host: ![]() ![]() #2: Chemical | ChemComp-ZN / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.67 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: Na/K phosphate, Hepes, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jun 8, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→60 Å / Num. all: 14806 / Num. obs: 14335 / % possible obs: 96.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0.5 / Redundancy: 3.3 % / Rsym value: 0.03 / Net I/σ(I): 27.4 |
Reflection shell | Resolution: 2→2.07 Å / Rsym value: 0.074 / % possible all: 85.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1R1T Resolution: 2→60 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2→60 Å
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Refine LS restraints |
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Xplor file |
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