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- PDB-1qwf: C-SRC SH3 DOMAIN COMPLEXED WITH LIGAND VSL12 -

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Basic information

Entry
Database: PDB / ID: 1qwf
TitleC-SRC SH3 DOMAIN COMPLEXED WITH LIGAND VSL12
Components
  • TYROSINE-PROTEIN KINASE TRANSFORMING PROTEIN SRC
  • VAL-SER-LEU-ALA-ARG-ARG-PRO-LEU-PRO-PRO-LEU-PRO
KeywordsCOMPLEX (SIGNAL TRANSDUCTION/PEPTIDE) / SRC SH3 DOMAIN / CLASS I LIGAND COMPLEX / COMPLEX (SIGNAL TRANSDUCTION-PEPTIDE) COMPLEX
Function / homology
Function and homology information


non-membrane spanning protein tyrosine kinase activity / non-specific protein-tyrosine kinase / ATP binding
Similarity search - Function
SH3 Domains / : / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / SH3 type barrels. / Src homology 3 domains / SH2 domain superfamily ...SH3 Domains / : / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / SH3 type barrels. / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / Tyrosine-protein kinase, catalytic domain / Tyrosine kinase, catalytic domain / Tyrosine protein kinases specific active-site signature. / Tyrosine-protein kinase, active site / Serine-threonine/tyrosine-protein kinase, catalytic domain / Protein tyrosine and serine/threonine kinase / Roll / Protein kinase, ATP binding site / Protein kinases ATP-binding region signature. / Protein kinase domain profile. / Protein kinase domain / Protein kinase-like domain superfamily / Mainly Beta
Similarity search - Domain/homology
Tyrosine-protein kinase transforming protein Src
Similarity search - Component
Biological speciesAvian sarcoma virus
MethodSOLUTION NMR
AuthorsFeng, S. / Chiyoshi, K. / Rickles, R.J. / Schreiber, S.L.
Citation
Journal: Proc.Natl.Acad.Sci.USA / Year: 1995
Title: Specific interactions outside the proline-rich core of two classes of Src homology 3 ligands.
Authors: Feng, S. / Kasahara, C. / Rickles, R.J. / Schreiber, S.L.
#1: Journal: Cell(Cambridge,Mass.) / Year: 1994
Title: Structural Basis for the Binding of Proline-Rich Peptides to SH3 Domains
Authors: Yu, H. / Chen, J.K. / Feng, S. / Dalgarno, D.C. / Brauer, A.W. / Schreiber, S.L.
#2: Journal: Science / Year: 1994
Title: Two Binding Orientations for Peptides to the Src SH3 Domain: Development of a General Model for SH3-Ligand Interactions
Authors: Feng, S. / Chen, J.K. / Yu, H. / Simon, J.A. / Schreiber, S.L.
#3: Journal: Science / Year: 1992
Title: Solution Structure of the SH3 Domain of Src and Identification of its Ligand-Binding Site
Authors: Yu, H. / Rosen, M.K. / Shin, T.B. / Seidel-Dugan, C. / Brugge, J.S. / Schreiber, S.L.
History
DepositionNov 9, 1995Processing site: BNL
Revision 1.0Mar 8, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Mar 2, 2022Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_ref_seq_dif.details
Revision 1.4May 22, 2024Group: Data collection / Category: chem_comp_atom / chem_comp_bond

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: TYROSINE-PROTEIN KINASE TRANSFORMING PROTEIN SRC
B: VAL-SER-LEU-ALA-ARG-ARG-PRO-LEU-PRO-PRO-LEU-PRO


Theoretical massNumber of molelcules
Total (without water)8,4222
Polymers8,4222
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)1 / -
Representative

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Components

#1: Protein TYROSINE-PROTEIN KINASE TRANSFORMING PROTEIN SRC / P60-SRC


Mass: 7104.749 Da / Num. of mol.: 1 / Fragment: SH3 DOMAIN
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Avian sarcoma virus / Genus: Alpharetrovirus / Description: GST-FUSION / Gene: CHICKEN / Plasmid: PGEX-2T / Gene (production host): CHICKEN / Production host: Escherichia coli (E. coli) / References: UniProt: P00525, EC: 2.7.1.112
#2: Protein/peptide VAL-SER-LEU-ALA-ARG-ARG-PRO-LEU-PRO-PRO-LEU-PRO / VSL12


Mass: 1317.622 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other

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Processing

Software
NameVersionClassification
X-PLOR3.1model building
X-PLOR3.1refinement
X-PLOR3.1phasing
NMR softwareName: X-PLOR / Version: 3.1 / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 1

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