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- PDB-1qsb: THE INTRODUCTION OF STRAIN AND ITS EFFECTS ON THE STRUCTURE AND S... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1qsb | ||||||
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Title | THE INTRODUCTION OF STRAIN AND ITS EFFECTS ON THE STRUCTURE AND STABILITY OF T4 LYSOZYME | ||||||
![]() | PROTEIN (LYSOZYME) | ||||||
![]() | HYDROLASE / STRAIN / STABILITY / MUTANT / T4 LYSOZYME | ||||||
Function / homology | ![]() viral release from host cell by cytolysis / peptidoglycan catabolic process / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / host cell cytoplasm / defense response to bacterium Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Liu, R. / Baase, W.A. / Matthews, B.W. | ||||||
![]() | ![]() Title: The introduction of strain and its effects on the structure and stability of T4 lysozyme. Authors: Liu, R. / Baase, W.A. / Matthews, B.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 47.7 KB | Display | ![]() |
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PDB format | ![]() | 33.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.5 KB | Display | ![]() |
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Full document | ![]() | 430.1 KB | Display | |
Data in XML | ![]() | 10.5 KB | Display | |
Data in CIF | ![]() | 14.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1qs5C ![]() 1qs9C ![]() 1qtbC ![]() 1qtcC ![]() 1qtdC ![]() 1qthC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18433.168 Da / Num. of mol.: 1 / Mutation: A98C, C54T, C97A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Chemical | #3: Chemical | ChemComp-HED / | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56.44 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.00 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, hanging drop / PH range low: 7.1 / PH range high: 6.3 | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→9.1 Å / Num. obs: 18718 / % possible obs: 90 % / Biso Wilson estimate: 17.3 Å2 / Rmerge(I) obs: 0.038 / Net I/σ(I): 9.698 |
Reflection shell | Highest resolution: 1.8 Å / Mean I/σ(I) obs: 1.76 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: CYS-FREE VERSION OF T4 LYSOZYME Resolution: 1.8→9.1 Å / σ(F): 0 / Stereochemistry target values: TNT PROTGEO
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Solvent computation | Solvent model: BABENET SCALING / Bsol: 171.2 Å2 / ksol: 0.544 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→9.1 Å
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Refine LS restraints |
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Refine LS restraints | *PLUS
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