+Open data
-Basic information
Entry | Database: PDB / ID: 1epy | ||||||
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Title | T4 LYSOZYME MUTANT, T21H/C54T/C97A/Q141H/T142H | ||||||
Components | LYSOZYME | ||||||
Keywords | HYDROLASE / metal binding | ||||||
Function / homology | Function and homology information viral release from host cell by cytolysis / peptidoglycan catabolic process / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / host cell cytoplasm / defense response to bacterium Similarity search - Function | ||||||
Biological species | Enterobacteria phage T4 (virus) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.85 Å | ||||||
Authors | Wray, J.W. / Baase, W.A. / Ostheimer, G.J. / Zhang, X.-J. / Matthews, B.W. | ||||||
Citation | Journal: Protein Eng. / Year: 2000 Title: Use of a non-rigid region in T4 lysozyme to design an adaptable metal-binding site. Authors: Wray, J.W. / Baase, W.A. / Ostheimer, G.J. / Zhang, X.J. / Matthews, B.W. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1989 Title: Stabilization of Phage T4 Lysozyme by Engineered Disulfide Bonds Authors: Matsumura, M. / Becktel, W.J. / Levitt, M. / Matthews, B.W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1epy.cif.gz | 47.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1epy.ent.gz | 33.3 KB | Display | PDB format |
PDBx/mmJSON format | 1epy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1epy_validation.pdf.gz | 431.4 KB | Display | wwPDB validaton report |
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Full document | 1epy_full_validation.pdf.gz | 435.7 KB | Display | |
Data in XML | 1epy_validation.xml.gz | 9.6 KB | Display | |
Data in CIF | 1epy_validation.cif.gz | 12.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ep/1epy ftp://data.pdbj.org/pub/pdb/validation_reports/ep/1epy | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18712.467 Da / Num. of mol.: 1 / Mutation: T21H,C54T,C97A,Q141H,T142H Source method: isolated from a genetically manipulated source Source: (gene. exp.) Enterobacteria phage T4 (virus) / Genus: T4-like viruses / Species: Enterobacteria phage T4 sensu lato / Production host: Escherichia coli (E. coli) / References: UniProt: P00720, lysozyme |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-CL / |
#4: Chemical | ChemComp-CO / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.86 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 12-16% PEG 8000, 10% isopropanol, sodium chloride, HEPES, 0.002 M cobalt chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 4K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: Oct 11, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.8 Å / Num. obs: 19318 |
-Processing
Software |
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Refinement | Resolution: 1.85→20 Å / Stereochemistry target values: TNT
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Refinement step | Cycle: LAST / Resolution: 1.85→20 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||
Refine LS restraints | *PLUS Type: t_plane_restr / Dev ideal: 0.019 |