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- PDB-1qq0: COBALT SUBSTITUTED CARBONIC ANHYDRASE FROM METHANOSARCINA THERMOPHILA -
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Open data
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Basic information
Entry | Database: PDB / ID: 1qq0 | ||||||
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Title | COBALT SUBSTITUTED CARBONIC ANHYDRASE FROM METHANOSARCINA THERMOPHILA | ||||||
![]() | CARBONIC ANHYDRASE | ||||||
![]() | LYASE / BETA-HELIX | ||||||
Function / homology | ![]() bicarbonate binding / sulfate binding / cobalt ion binding / carbonic anhydrase / carbonate dehydratase activity / zinc ion binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Iverson, T.M. / Alber, B.E. / Kisker, C. / Ferry, J.G. / Rees, D.C. | ||||||
![]() | ![]() Title: A closer look at the active site of gamma-class carbonic anhydrases: high-resolution crystallographic studies of the carbonic anhydrase from Methanosarcina thermophila. Authors: Iverson, T.M. / Alber, B.E. / Kisker, C. / Ferry, J.G. / Rees, D.C. #1: ![]() Title: A Left-Handed Beta-Helix Revealed by the Crystal Structure of a Carbonic Anhydrase from the Archaeon Methanosarcina Thermophila Authors: Kisker, C. / Schindelin, H. / Alber, B. / Ferry, J. / Rees, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 54.7 KB | Display | ![]() |
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PDB format | ![]() | 38.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 364.7 KB | Display | ![]() |
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Full document | ![]() | 366.7 KB | Display | |
Data in XML | ![]() | 6 KB | Display | |
Data in CIF | ![]() | 8.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 26429.412 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CO / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.76 Å3/Da / Density % sol: 30.13 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5.8 Details: PEG 8000, AMMONIUM SULFATE, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 22K | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6.2 | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: May 5, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.08 Å / Relative weight: 1 |
Reflection | Resolution: 1.76→19.4 Å / Num. all: 95188 / Num. obs: 18449 / % possible obs: 98.7 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Redundancy: 5.2 % / Biso Wilson estimate: 22 Å2 / Rmerge(I) obs: 0.054 / Net I/σ(I): 21 |
Reflection shell | Resolution: 1.76→1.82 Å / Redundancy: 3 % / Rmerge(I) obs: 0.283 / % possible all: 98.7 |
Reflection | *PLUS Num. measured all: 95188 |
Reflection shell | *PLUS % possible obs: 98.7 % / Mean I/σ(I) obs: 4.2 |
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Processing
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Refinement | Resolution: 1.76→19.4 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: STANDARD CCP4 DICTIONARY
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Refinement step | Cycle: LAST / Resolution: 1.76→19.4 Å
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Software | *PLUS Name: REFMAC / Classification: refinement | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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