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- PDB-1qov: PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M260 REPLACED WITH... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1qov | ||||||
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Title | PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M260 REPLACED WITH TRP (CHAIN M, A260W) | ||||||
![]() | (PHOTOSYNTHETIC REACTION ...) x 3 | ||||||
![]() | PHOTOSYNTHETIC REACTION CENTER / TRANSMEMBRANE / ELECTRON TRANSPORT / PHOTOSYNTHESIS / CARDIOLIPIN / MEMBRANE PROTEIN | ||||||
Function / homology | ![]() : / plasma membrane-derived chromatophore membrane / plasma membrane light-harvesting complex / bacteriochlorophyll binding / photosynthesis, light reaction / electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity / photosynthetic electron transport in photosystem II / membrane => GO:0016020 / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | McAuley, K.E. / Fyfe, P.K. / Ridge, J.P. / Isaacs, N.W. / Cogdell, R.J. / Jones, M.R. | ||||||
![]() | ![]() Title: Structural Details of an Interaction between Cardiolipin and an Integral Membrane Protein Authors: Mcauley, K.E. / Fyfe, P.K. / Ridge, J.P. / Isaacs, N.W. / Cogdell, R.J. / Jones, M.R. #1: ![]() Title: Structural Studies of Wild-Type and Mutant Reaction Centers from an Antenna-Deficient Strain of Rhodobacter Sphaeroides: Monitoring the Optical Properties of the Complex from Bacterial Cell to Crystal Authors: Mcauley-Hecht, K.E. / Fyfe, P.K. / Ridge, J.P. / Prince, S.M. / Hunter, C.N. / Isaacs, N.W. / Cogdell, R.J. / Jones, M.R. #2: Journal: Biochemistry / Year: 1992 Title: Construction and Characterization of a Mutant of Rhodobacter Sphaeroides with the Reaction Center as the Sole Pigment-Protein Complex Authors: Jones, M.R. / Visschers, R.W. / Van Grondelle, R. / Hunter, C.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 204 KB | Display | ![]() |
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PDB format | ![]() | 156.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 905.9 KB | Display | ![]() |
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Full document | ![]() | 953.2 KB | Display | |
Data in XML | ![]() | 25.5 KB | Display | |
Data in CIF | ![]() | 36.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-PHOTOSYNTHETIC REACTION ... , 3 types, 3 molecules HLM
#1: Protein | Mass: 28066.322 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Protein | Mass: 31346.389 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Protein | Mass: 34513.676 Da / Num. of mol.: 1 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
-Non-polymers , 8 types, 261 molecules ![](data/chem/img/BCL.gif)
![](data/chem/img/U10.gif)
![](data/chem/img/BPH.gif)
![](data/chem/img/FE2.gif)
![](data/chem/img/SPN.gif)
![](data/chem/img/CDL.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/U10.gif)
![](data/chem/img/BPH.gif)
![](data/chem/img/FE2.gif)
![](data/chem/img/SPN.gif)
![](data/chem/img/CDL.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-BCL / #5: Chemical | ChemComp-U10 / | #6: Chemical | #7: Chemical | ChemComp-FE2 / | #8: Chemical | ChemComp-SPN / | #9: Chemical | ChemComp-CDL / | #10: Chemical | ChemComp-CL / | #11: Water | ChemComp-HOH / | |
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-Details
Compound details | CHAIN M HAS A M260W MUTATION |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.5 Å3/Da / Density % sol: 76 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8 / Details: pH 8.00 | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion, sitting drop | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Mar 15, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→30 Å / Num. obs: 124853 / % possible obs: 97.6 % / Redundancy: 4.2 % / Biso Wilson estimate: 24.06 Å2 / Rmerge(I) obs: 0.057 |
Reflection shell | Resolution: 2.1→2.15 Å / Redundancy: 1.6 % / Rmerge(I) obs: 0.266 / % possible all: 78.6 |
Reflection shell | *PLUS % possible obs: 78.6 % |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: WILD-TYPE RHODOBACTER SPHAEROIDES COORDINATES (UNPUBLISHED DATA) Resolution: 2.1→30 Å / SU B: 2.5 / SU ML: 0.066 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.104 / ESU R Free: 0.099 Details: X-PLOR (VERSION 3.1) BULK SOLVENT CORRECTION APPLIED
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Displacement parameters | Biso mean: 40.02 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→30 Å
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Refine LS restraints |
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