+Open data
-Basic information
Entry | Database: PDB / ID: 1qog | ||||||
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Title | FERREDOXIN MUTATION S47A | ||||||
Components | FERREDOXIN | ||||||
Keywords | ELECTRON TRANSPORT / IRON-SULFUR / FERREDOXIN | ||||||
Function / homology | Function and homology information 2 iron, 2 sulfur cluster binding / electron transfer activity / metal ion binding Similarity search - Function | ||||||
Biological species | Nostoc sp. (bacteria) | ||||||
Method | X-RAY DIFFRACTION / DIFFERENCE FOURIER / Resolution: 1.8 Å | ||||||
Authors | Holden, H.M. / Benning, M.M. | ||||||
Citation | Journal: Biochemistry / Year: 1997 Title: Structure-function relationships in Anabaena ferredoxin: correlations between X-ray crystal structures, reduction potentials, and rate constants of electron transfer to ferredoxin:NADP+ ...Title: Structure-function relationships in Anabaena ferredoxin: correlations between X-ray crystal structures, reduction potentials, and rate constants of electron transfer to ferredoxin:NADP+ reductase for site-specific ferredoxin mutants. Authors: Hurley, J.K. / Weber-Main, A.M. / Stankovich, M.T. / Benning, M.M. / Thoden, J.B. / Vanhooke, J.L. / Holden, H.M. / Chae, Y.K. / Xia, B. / Cheng, H. / Markley, J.L. / Martinez-Julvez, M. / ...Authors: Hurley, J.K. / Weber-Main, A.M. / Stankovich, M.T. / Benning, M.M. / Thoden, J.B. / Vanhooke, J.L. / Holden, H.M. / Chae, Y.K. / Xia, B. / Cheng, H. / Markley, J.L. / Martinez-Julvez, M. / Gomez-Moreno, C. / Schmeits, J.L. / Tollin, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qog.cif.gz | 51.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qog.ent.gz | 37.8 KB | Display | PDB format |
PDBx/mmJSON format | 1qog.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qo/1qog ftp://data.pdbj.org/pub/pdb/validation_reports/qo/1qog | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 10689.624 Da / Num. of mol.: 2 / Mutation: S47A Source method: isolated from a genetically manipulated source Details: 2FE-2S FERREDOXIN / Source: (gene. exp.) Nostoc sp. (bacteria) / Strain: PCC 7120 / Plasmid: PAN662 / Production host: Escherichia coli (E. coli) / Strain (production host): JM109 / References: UniProt: P0A3C7 #2: Chemical | ChemComp-SO4 / | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.77 % | ||||||||||||||||||||
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Crystal grow | *PLUS pH: 5.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 269 K |
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Diffraction source | Wavelength: 1.5418 |
Detector | Type: SIEMENS / Detector: AREA DETECTOR |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→25 Å / Num. obs: 19030 / % possible obs: 92 % / Observed criterion σ(I): 0 / Redundancy: 2.3 % / Rmerge(I) obs: 0.044 / Net I/σ(I): 24.6 |
Reflection shell | Resolution: 1.8→1.88 Å / Redundancy: 3 % / Rmerge(I) obs: 0.13 / Mean I/σ(I) obs: 4.1 / % possible all: 82 |
Reflection | *PLUS Num. measured all: 43417 |
Reflection shell | *PLUS |
-Processing
Software |
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Refinement | Method to determine structure: DIFFERENCE FOURIER / Resolution: 1.8→25 Å
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Refinement step | Cycle: LAST / Resolution: 1.8→25 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Rfactor all: 0.182 / Rfactor Rwork: 0.182 / Highest resolution: 1.8 Å | ||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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