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- PDB-1qhl: CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF MUKB AT 2.2A RESOLUTION -
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Open data
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Basic information
Entry | Database: PDB / ID: 1qhl | ||||||
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Title | CRYSTAL STRUCTURE OF THE N-TERMINAL DOMAIN OF MUKB AT 2.2A RESOLUTION | ||||||
![]() | PROTEIN (CELL DIVISION PROTEIN MUKB) | ||||||
![]() | CELL DIVISION PROTEIN / MUKB / SMC / CHROMOSOME PARTITIONING | ||||||
Function / homology | ![]() condensin complex / nucleoid / sister chromatid cohesion / chromosome condensation / chromosome segregation / DNA replication / cell division / GTP binding / DNA binding / ATP binding ...condensin complex / nucleoid / sister chromatid cohesion / chromosome condensation / chromosome segregation / DNA replication / cell division / GTP binding / DNA binding / ATP binding / identical protein binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | van den Ent, F. / Lowe, J. | ||||||
![]() | ![]() Title: Crystal structure of the N-terminal domain of MukB: a protein involved in chromosome partitioning. Authors: van den Ent, F. / Lockhart, A. / Kendrick-Jones, J. / Lowe, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.8 KB | Display | ![]() |
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PDB format | ![]() | 37.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 424.2 KB | Display | ![]() |
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Full document | ![]() | 432 KB | Display | |
Data in XML | ![]() | 11.1 KB | Display | |
Data in CIF | ![]() | 14.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25246.979 Da / Num. of mol.: 1 / Fragment: N-TERMINAL DOMAIN Source method: isolated from a genetically manipulated source Details: THIS PROTEIN IS LINKED TO A SYNTHETIC C-TERMINAL HIS TAG (GSHHHHHH). THE TAG WAS NOT SEEN IN THE ELECTRON DENSITY. Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.22 Å3/Da / Density % sol: 45 % | ||||||||||||||||||||
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Crystal grow | pH: 4.9 Details: 0.1M NA-CITRATE PH 4.9, 18-22% PEG 600, 50MG/ML, DROPS 1+1 | ||||||||||||||||||||
Crystal | *PLUS | ||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, sitting drop | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() | |||||||||||||||
Detector | Type: MARRESEARCH / Detector: CCD / Date: Apr 1, 1999 | |||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.2→40 Å / Num. obs: 382097 / % possible obs: 99.6 % / Redundancy: 13.1 % / Biso Wilson estimate: 30.9 Å2 / Rmerge(I) obs: 0.091 / Net I/σ(I): 5 | |||||||||||||||
Reflection shell | Resolution: 2.2→2.34 Å / Redundancy: 11.3 % / Rmerge(I) obs: 0.263 / Mean I/σ(I) obs: 2.9 / % possible all: 97.3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 30.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.2 Å / Lowest resolution: 10 Å / σ(F): 0 / % reflection Rfree: 5 % / Rfactor obs: 0.217 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 30.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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