+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1qgb | ||||||
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タイトル | SOLUTION STRUCTURE OF THE N-TERMINAL F1 MODULE PAIR FROM HUMAN FIBRONECTIN | ||||||
要素 | PROTEIN (FIBRONECTIN) | ||||||
キーワード | CELL ADHESION (細胞接着) / FIBRONECTIN TYPE 1 MODULE PAIR | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of monocyte activation / calcium-independent cell-matrix adhesion / negative regulation of transforming growth factor beta production / Fibronectin matrix formation / Extracellular matrix organization / positive regulation of substrate-dependent cell migration, cell attachment to substrate / neural crest cell migration involved in autonomic nervous system development / peptidase activator activity / fibrinogen complex / peptide cross-linking ...negative regulation of monocyte activation / calcium-independent cell-matrix adhesion / negative regulation of transforming growth factor beta production / Fibronectin matrix formation / Extracellular matrix organization / positive regulation of substrate-dependent cell migration, cell attachment to substrate / neural crest cell migration involved in autonomic nervous system development / peptidase activator activity / fibrinogen complex / peptide cross-linking / integrin activation / ALK mutants bind TKIs / cell-substrate junction assembly / biological process involved in interaction with symbiont / Molecules associated with elastic fibres / proteoglycan binding / extracellular matrix structural constituent / MET activates PTK2 signaling / Syndecan interactions / p130Cas linkage to MAPK signaling for integrins / endodermal cell differentiation / GRB2:SOS provides linkage to MAPK signaling for Integrins / Non-integrin membrane-ECM interactions / 基底膜 / endoplasmic reticulum-Golgi intermediate compartment / ECM proteoglycans / positive regulation of axon extension / Integrin cell surface interactions / collagen binding / Degradation of the extracellular matrix / Integrin signaling / substrate adhesion-dependent cell spreading / cell-matrix adhesion / 細胞外マトリックス / regulation of ERK1 and ERK2 cascade / platelet alpha granule lumen / acute-phase response / integrin-mediated signaling pathway / Cell surface interactions at the vascular wall / Post-translational protein phosphorylation / regulation of protein phosphorylation / Signaling by high-kinase activity BRAF mutants / wound healing / MAP2K and MAPK activation / Signaling by ALK fusions and activated point mutants / response to wounding / GPER1 signaling / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) / positive regulation of fibroblast proliferation / Signaling by BRAF and RAF1 fusions / integrin binding / Platelet degranulation / heparin binding / nervous system development / heart development / regulation of cell shape / Interleukin-4 and Interleukin-13 signaling / 血管新生 / collagen-containing extracellular matrix / protease binding / blood microparticle / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / 細胞接着 / apical plasma membrane / 小胞体 / signaling receptor binding / positive regulation of cell population proliferation / positive regulation of gene expression / extracellular space / extracellular exosome / extracellular region / identical protein binding / 細胞膜 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Potts, J.R. / Bright, J.R. / Bolton, D. / Pickford, A.R. / Campbell, I.D. | ||||||
引用 | ジャーナル: Biochemistry / 年: 1999 タイトル: Solution structure of the N-terminal F1 module pair from human fibronectin. 著者: Potts, J.R. / Bright, J.R. / Bolton, D. / Pickford, A.R. / Campbell, I.D. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1qgb.cif.gz | 664.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1qgb.ent.gz | 554.6 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1qgb.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/qg/1qgb ftp://data.pdbj.org/pub/pdb/validation_reports/qg/1qgb | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 10524.674 Da / 分子数: 1 / 断片: N-TERMINAL F1 MODULE PAIR / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Pichia pastoris (菌類) / 株 (発現宿主): GS115 / 参照: UniProt: P02751 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||
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NMR実験 |
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NMR実験の詳細 | Text: THE STRUCTURE WAS DETERMINED USING NMR SPECTROSCOPY ON UNIFORMLY 15N-LABELLED 1F1-2F1 |
-試料調製
詳細 | タイプ: solution / 内容: 2.2 mM [U-15N] 1F1-2F1, 90% H2O/10% D2O / Label: sample_1 / 溶媒系: 90% H2O/10% D2O |
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試料 | 濃度: 2.2 mM / 構成要素: 1F1-2F1 / Isotopic labeling: [U-15N] |
試料状態 | pH: 4.0 / 温度: 310 K |
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 1 詳細: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: LOW ENERGY AND AGREEMENT WITH EXPERIMENTAL RESTRAINTS 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 24 |