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Yorodumi- PDB-1qfv: HISTAMINE BINDING PROTEIN FROM FEMALE BROWN EAR RHIPICEPHALUS APP... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1qfv | ||||||
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Title | HISTAMINE BINDING PROTEIN FROM FEMALE BROWN EAR RHIPICEPHALUS APPENDICULATUS | ||||||
Components | PROTEIN (FEMALE-SPECIFIC HISTAMINE BINDING PROTEIN 2) | ||||||
Keywords | LIGAND BINDING PROTEIN / LIPOCALIN | ||||||
Function / homology | Function and homology information amine binding / symbiont-mediated perturbation of host defenses / extracellular region Similarity search - Function | ||||||
Biological species | Rhipicephalus appendiculatus (arthropod) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 1.36 Å | ||||||
Authors | Paesen, G.C. / Adams, P.L. / Harlos, K. / Nuttal, P.A. / Stuart, D.I. | ||||||
Citation | Journal: Mol.Cell / Year: 1999 Title: Tick histamine-binding proteins: isolation, cloning, and three-dimensional structure. Authors: Paesen, G.C. / Adams, P.L. / Harlos, K. / Nuttall, P.A. / Stuart, D.I. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1qfv.cif.gz | 91.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1qfv.ent.gz | 70.6 KB | Display | PDB format |
PDBx/mmJSON format | 1qfv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1qfv_validation.pdf.gz | 385.9 KB | Display | wwPDB validaton report |
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Full document | 1qfv_full_validation.pdf.gz | 390.7 KB | Display | |
Data in XML | 1qfv_validation.xml.gz | 9.1 KB | Display | |
Data in CIF | 1qfv_validation.cif.gz | 15.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qf/1qfv ftp://data.pdbj.org/pub/pdb/validation_reports/qf/1qfv | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 19810.441 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rhipicephalus appendiculatus (arthropod) Organ: SALIVARY GLAND / Plasmid: PACC129.1 / Genus (production host): Spodoptera / Cell line (production host): SPODOPTERA / Production host: Spodoptera (butterflies/moths) / Strain (production host): SF21 / References: UniProt: O77421 #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.84 Å3/Da / Density % sol: 56.62 % | |||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.50 | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6.5 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X12C / Wavelength: 1.072 |
Detector | Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.072 Å / Relative weight: 1 |
Reflection | Resolution: 1.36→20 Å / Num. obs: 108735 / % possible obs: 74.8 % / Redundancy: 2.7 % / Rsym value: 0.063 |
Reflection | *PLUS Highest resolution: 1.36 Å / Lowest resolution: 20 Å / % possible obs: 74.8 % / Redundancy: 2.7 % / Rmerge(I) obs: 0.183 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 1.36→20 Å / σ(F): 0
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Refinement step | Cycle: LAST / Resolution: 1.36→20 Å
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Refine LS restraints |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.36 Å / Lowest resolution: 20 Å / Rfactor obs: 0.193 / Rfactor Rfree: 0.213 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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