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- PDB-1qfj: CRYSTAL STRUCTURE OF NAD(P)H:FLAVIN OXIDOREDUCTASE FROM ESCHERICH... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1qfj | ||||||
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Title | CRYSTAL STRUCTURE OF NAD(P)H:FLAVIN OXIDOREDUCTASE FROM ESCHERICHIA COLI | ||||||
![]() | PROTEIN (FLAVIN REDUCTASE) | ||||||
![]() | OXIDOREDUCTASE / RIBOFLAVIN / FLAVIN REDUCTASE / FERREDOXIN REDUCTASE SUPERFAMILY | ||||||
Function / homology | ![]() riboflavin reductase [NAD(P)H] / riboflavin reductase (NAD(P)H) activity / protein repair / riboflavin reductase (NADPH) activity / : / monoatomic ion transport / enzyme activator activity / response to oxidative stress / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ingelman, M. / Ramaswamy, S. / Niviere, V. / Fontecave, M. / Eklund, H. | ||||||
![]() | ![]() Title: Crystal structure of NAD(P)H:flavin oxidoreductase from Escherichia coli. Authors: Ingelman, M. / Ramaswamy, S. / Niviere, V. / Fontecave, M. / Eklund, H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 189.4 KB | Display | ![]() |
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PDB format | ![]() | 154.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 453.2 KB | Display | ![]() |
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Full document | ![]() | 477.5 KB | Display | |
Data in XML | ![]() | 38.7 KB | Display | |
Data in CIF | ![]() | 53.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 26143.791 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: DISULFIDE LINKS BETWEEN MOLECULES A AND D AS WELL AS B AND C RESPECTIVELY Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-GOL / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 51 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 Details: 15 % PEG 4000, 0.5 M NACL, 100 MM BIS-TRIS PROPANE PH 6.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jun 1, 1995 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.864 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→20 Å / Num. all: 54692 / Num. obs: 54692 / % possible obs: 93.2 % / Observed criterion σ(I): 0 / Redundancy: 6.8 % / Rmerge(I) obs: 0.064 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 2.2→2.24 Å / Redundancy: 6 % / Rmerge(I) obs: 0.235 / Mean I/σ(I) obs: 3.9 / % possible all: 88.2 |
Reflection | *PLUS Num. measured all: 626153 |
Reflection shell | *PLUS % possible obs: 88.2 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 35.4 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→20 Å
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Refine LS restraints |
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Software | *PLUS Name: REFMAC / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |