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Open data
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Basic information
Entry | Database: PDB / ID: 1q1u | ||||||
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Title | Crystal structure of human FHF1b (FGF12b) | ||||||
![]() | fibroblast growth factor homologous factor 1 | ||||||
![]() | HORMONE/GROWTH FACTOR / FGF-12 / Human / crystal structure / HORMONE-GROWTH FACTOR COMPLEX | ||||||
Function / homology | ![]() regulation of voltage-gated sodium channel activity / regulation of neuronal action potential / regulation of sodium ion transmembrane transport / regulation of sodium ion transmembrane transporter activity / positive regulation of sodium ion transport / cardiac muscle cell action potential involved in contraction / neuromuscular process / Phase 0 - rapid depolarisation / sodium channel regulator activity / JNK cascade ...regulation of voltage-gated sodium channel activity / regulation of neuronal action potential / regulation of sodium ion transmembrane transport / regulation of sodium ion transmembrane transporter activity / positive regulation of sodium ion transport / cardiac muscle cell action potential involved in contraction / neuromuscular process / Phase 0 - rapid depolarisation / sodium channel regulator activity / JNK cascade / adult locomotory behavior / growth factor activity / cell-cell signaling / nervous system development / heparin binding / heart development / chemical synaptic transmission / transmembrane transporter binding / synapse / signal transduction / extracellular space / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Olsen, S.K. / Garbi, M. / Zampieri, N. / Eliseenkova, A.V. / Ornitz, D.M. / Goldfarb, M. / Mohammadi, M. | ||||||
![]() | ![]() Title: Fibroblast growth factor (FGF) homologous factors share structural but not functional homology with FGFs Authors: Olsen, S.K. / Garbi, M. / Zampieri, N. / Eliseenkova, A.V. / Ornitz, D.M. / Goldfarb, M. / Mohammadi, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 41 KB | Display | ![]() |
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PDB format | ![]() | 27.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 376.9 KB | Display | ![]() |
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Full document | ![]() | 377.8 KB | Display | |
Data in XML | ![]() | 4.2 KB | Display | |
Data in CIF | ![]() | 6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1ihkS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 16365.646 Da / Num. of mol.: 1 / Fragment: residues 1-144 / Mutation: C144A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||
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#2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.8 Å3/Da / Density % sol: 31.7 % | ||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 20% PEG 400, 200mM ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 90 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Oct 30, 2002 |
Radiation | Monochromator: Kohzu double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.920218 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→30 Å / Num. all: 13559 / Num. obs: 13171 / % possible obs: 97 % / Observed criterion σ(I): 0 / Rsym value: 0.058 / Net I/σ(I): 7.03 |
Reflection shell | Resolution: 1.7→1.76 Å / % possible obs: 85 % / % possible all: 85 |
Reflection | *PLUS Highest resolution: 1.7 Å / Num. measured all: 93909 / Rmerge(I) obs: 0.058 |
Reflection shell | *PLUS Highest resolution: 1.7 Å / Rmerge(I) obs: 0.365 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ID 1IHK Resolution: 1.7→25 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.7→25 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 1.7 Å / % reflection Rfree: 10 % / Rfactor Rfree: 0.24 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS |