+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 1q0w | ||||||
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タイトル | Solution structure of Vps27 amino-terminal UIM-ubiquitin complex | ||||||
要素 |
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キーワード | TRANSPORT PROTEIN (運搬体タンパク質) / PROTEIN-PROTEIN COMPLEX | ||||||
機能・相同性 | 機能・相同性情報 microlipophagy / positive regulation of protein maturation / : / : / Metalloprotease DUBs / : / : / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Regulation of PTEN localization / ER Quality Control Compartment (ERQC) ...microlipophagy / positive regulation of protein maturation / : / : / Metalloprotease DUBs / : / : / Synthesis of active ubiquitin: roles of E1 and E2 enzymes / Regulation of PTEN localization / ER Quality Control Compartment (ERQC) / Interleukin-1 signaling / Negative regulators of DDX58/IFIH1 signaling / E3 ubiquitin ligases ubiquitinate target proteins / Translesion Synthesis by POLH / : / UCH proteinases / Regulation of PTEN stability and activity / Aggrephagy / ESCRT-0 complex / Regulation of TP53 Degradation / CDK-mediated phosphorylation and removal of Cdc6 / FBXL7 down-regulates AURKA during mitotic entry and in early mitosis / Ubiquitin Mediated Degradation of Phosphorylated Cdc25A / Translesion synthesis by POLK / Translesion synthesis by POLI / Peroxisomal protein import / Orc1 removal from chromatin / Translesion synthesis by REV1 / ABC-family proteins mediated transport / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / microautophagy / Endosomal Sorting Complex Required For Transport (ESCRT) / ATP export / protein retention in Golgi apparatus / MAPK6/MAPK4 signaling / protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / protein targeting to vacuole / Antigen processing: Ubiquitination & Proteasome degradation / multivesicular body sorting pathway / late endosome to vacuole transport / ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway / phosphatidylinositol-3-phosphate binding / vacuolar membrane / Formation of the ternary complex, and subsequently, the 43S complex / Ribosomal scanning and start codon recognition / Formation of TC-NER Pre-Incision Complex / Ub-specific processing proteases / Dual incision in TC-NER / Major pathway of rRNA processing in the nucleolus and cytosol / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Formation of a pool of free 40S subunits / Gap-filling DNA repair synthesis and ligation in TC-NER / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / L13a-mediated translational silencing of Ceruloplasmin expression / protein secretion / ribosomal large subunit export from nucleus / ubiquitin binding / modification-dependent protein catabolic process / ribosomal large subunit assembly / protein tag activity / リボソーム生合成 / cytoplasmic translation / cytosolic large ribosomal subunit / protein ubiquitination / endosome membrane / エンドソーム / structural constituent of ribosome / protein heterodimerization activity / protein domain specific binding / ubiquitin protein ligase binding / protein-containing complex / metal ion binding / 細胞核 / 細胞質基質 / 細胞質 類似検索 - 分子機能 | ||||||
生物種 | Saccharomyces cerevisiae (パン酵母) | ||||||
手法 | 溶液NMR / TORSION ANGLE DYNAMICS, SIMULATED ANNEALING | ||||||
データ登録者 | Swanson, K.A. / Kang, R.S. / Stamenova, S.D. / Hicke, L. / Radhakrishnan, I. | ||||||
引用 | ジャーナル: Embo J. / 年: 2003 タイトル: Solution Structure of Vps27 UIM-Ubiquitin Complex Important for Endosomal Sorting and Receptor Downregulation 著者: Swanson, K.A. / Kang, R.S. / Stamenova, S.D. / Hicke, L. / Radhakrishnan, I. | ||||||
履歴 |
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Remark 650 | HELIX Author determined secondary structure | ||||||
Remark 700 | SHEET Author determined secondary structure |
-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 1q0w.cif.gz | 627.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb1q0w.ent.gz | 524.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 1q0w.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/q0/1q0w ftp://data.pdbj.org/pub/pdb/validation_reports/q0/1q0w | HTTPS FTP |
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-関連構造データ
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質・ペプチド | 分子量: 2824.103 Da / 分子数: 1 / 断片: Amino-terminal UIM, residues 256 to 278 / 由来タイプ: 合成 詳細: The sequence was synthesized using a peptide synthesizer. The sequence contains a non-native tyrosine residue at the amino-terminus (residue number 255). The sequence occurs naturally in S. Cerevisiae. 参照: UniProt: P40343 |
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#2: タンパク質 | 分子量: 8568.769 Da / 分子数: 1 / 由来タイプ: 組換発現 由来: (組換発現) Saccharomyces cerevisiae (パン酵母) 遺伝子: (UBI1 OR RPL40A OR YIL148W) AND (UBI2 OR RPL40B OR YKR094C) AND (UBI3 OR RPS31 OR YLR167W OR L9470.14) AND (UBI4 OR SCD2 OR YLL039C) プラスミド: pET3-a / 生物種 (発現宿主): Escherichia coli / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: P61864, UniProt: P0CG63*PLUS |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料状態 | イオン強度: 20 mM sodium phosphate, pH 6.0, 0.2% NaN3 / pH: 6.0 / 圧: 1 atm / 温度: 298 K | |||||||||
結晶化 | *PLUS 手法: その他 / 詳細: NMR |
-NMR測定
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M |
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放射波長 | 相対比: 1 |
NMRスペクトロメーター | タイプ: Varian INOVA / 製造業者: Varian / モデル: INOVA / 磁場強度: 600 MHz |
-解析
NMR software |
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精密化 | 手法: TORSION ANGLE DYNAMICS, SIMULATED ANNEALING / ソフトェア番号: 1 詳細: THE STRUCTURES ARE BASED ON A TOTAL OF 2138 DISTANCE RESTRAINTS, INCLUDING 2062 NOE-DERIVED DISTANCE RESTRAINTS [1625 UNAMBIGUOUS AND 437 AMBIGUOUS RESTRAINTS], 76 HYDROGEN BONDING DISTANCE ...詳細: THE STRUCTURES ARE BASED ON A TOTAL OF 2138 DISTANCE RESTRAINTS, INCLUDING 2062 NOE-DERIVED DISTANCE RESTRAINTS [1625 UNAMBIGUOUS AND 437 AMBIGUOUS RESTRAINTS], 76 HYDROGEN BONDING DISTANCE RESTRAINTS, AND 112 TORSION ANGLE RESTRAINTS. | ||||||||||||||||||||
代表構造 | 選択基準: closest to the average | ||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: The submitted conformers are the 20 structures with the lowest restraint energies, restraint violations, and RMS deviations from ideal covalent geometry 計算したコンフォーマーの数: 80 / 登録したコンフォーマーの数: 20 |