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Open data
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Basic information
Entry | Database: PDB / ID: 1py9 | ||||||
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Title | The crystal structure of an autoantigen in multiple sclerosis | ||||||
![]() | Myelin-oligodendrocyte glycoprotein | ||||||
![]() | IMMUNE SYSTEM / myelin sheath / multiple sclerosis / receptor / immunoglobulin / anti-parallel dimer | ||||||
Function / homology | ![]() regulation of immune response / regulation of cytokine production / myelin sheath / T cell receptor signaling pathway / membrane => GO:0016020 / cell adhesion / membrane raft / external side of plasma membrane / signaling receptor binding / cell surface Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Clements, C.S. / Reid, H.H. / Beddoe, T. / Tynan, F.E. / Perugini, M.A. / Johns, T.G. / Bernard, C.C. / Rossjohn, J. | ||||||
![]() | ![]() Title: The crystal structure of myelin oligodendrocyte glycoprotein, a key autoantigen in multiple sclerosis Authors: Clements, C.S. / Reid, H.H. / Beddoe, T. / Tynan, F.E. / Perugini, M.A. / Johns, T.G. / Bernard, C.C. / Rossjohn, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 41.2 KB | Display | ![]() |
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PDB format | ![]() | 28 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 434.4 KB | Display | ![]() |
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Full document | ![]() | 435.5 KB | Display | |
Data in XML | ![]() | 9.5 KB | Display | |
Data in CIF | ![]() | 13.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | monomer in the asymmtric unit. However a crystallographic, head-to-tail dimer is present |
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Components
#1: Protein | Mass: 13277.826 Da / Num. of mol.: 1 / Fragment: extracellular domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 44.87 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 4.6 Details: PEG, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K | |||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8 / Method: vapor diffusion, hanging drop | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Aug 24, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.8→100 Å / Num. all: 13229 / Num. obs: 13229 / % possible obs: 99 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.5 % / Rmerge(I) obs: 0.065 / Rsym value: 0.069 / Net I/σ(I): 6.9 |
Reflection shell | Resolution: 1.8→1.9 Å / Rmerge(I) obs: 0.246 / Rsym value: 0.018 / % possible all: 100 |
Reflection | *PLUS % possible obs: 99 % / Num. measured all: 33022 / Rmerge(I) obs: 0.065 |
Reflection shell | *PLUS % possible obs: 100 % / Mean I/σ(I) obs: 1.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: B7.2 Resolution: 1.8→100 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.8→100 Å
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Refinement | *PLUS Lowest resolution: 50 Å / % reflection Rfree: 3 % | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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