[English] 日本語
![](img/lk-miru.gif)
- PDB-1pv3: NMR Solution Structure of the Avian FAT-domain of Focal Adhesion ... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1pv3 | ||||||
---|---|---|---|---|---|---|---|
Title | NMR Solution Structure of the Avian FAT-domain of Focal Adhesion Kinase | ||||||
![]() | Focal adhesion kinase 1 | ||||||
![]() | TRANSFERASE / Focal Adhesion Kinase / Helix Bundle / FAT-Domain | ||||||
Function / homology | ![]() Apoptotic cleavage of cellular proteins / NCAM signaling for neurite out-growth / RHO GTPases Activate WASPs and WAVEs / RAF/MAP kinase cascade / Regulation of actin dynamics for phagocytic cup formation / radial glia-guided pyramidal neuron migration / negative regulation of protein autophosphorylation / calcium-dependent cysteine-type endopeptidase activity / positive regulation of substrate-dependent cell migration, cell attachment to substrate / Integrin signaling ...Apoptotic cleavage of cellular proteins / NCAM signaling for neurite out-growth / RHO GTPases Activate WASPs and WAVEs / RAF/MAP kinase cascade / Regulation of actin dynamics for phagocytic cup formation / radial glia-guided pyramidal neuron migration / negative regulation of protein autophosphorylation / calcium-dependent cysteine-type endopeptidase activity / positive regulation of substrate-dependent cell migration, cell attachment to substrate / Integrin signaling / GRB2:SOS provides linkage to MAPK signaling for Integrins / DCC mediated attractive signaling / MET activates PTK2 signaling / Extra-nuclear estrogen signaling / EPHB-mediated forward signaling / p130Cas linkage to MAPK signaling for integrins / VEGFA-VEGFR2 Pathway / angiogenesis involved in wound healing / signal complex assembly / response to pH / negative regulation of cell-substrate adhesion / wound healing, spreading of cells / positive regulation of focal adhesion assembly / negative regulation of anoikis / positive regulation of protein tyrosine kinase activity / regulation of cell adhesion / response to muscle stretch / ciliary basal body / molecular function activator activity / actin filament organization / non-specific protein-tyrosine kinase / non-membrane spanning protein tyrosine kinase activity / epidermal growth factor receptor signaling pathway / sarcolemma / integrin binding / positive regulation of protein binding / cell cortex / protein tyrosine kinase activity / protease binding / protein autophosphorylation / dendritic spine / positive regulation of cell migration / focal adhesion / centrosome / positive regulation of cell population proliferation / perinuclear region of cytoplasm / ATP binding / identical protein binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / distance geometry simulated annealing | ||||||
![]() | Prutzman, K.C. / Gao, G. / King, M.L. / Iyer, V.V. / Mueller, G.A. / Schaller, M.D. / Campbell, S.L. | ||||||
![]() | ![]() Title: The Focal Adhesion Targeting Domain of Focal Adhesion Kinase Contains a Hinge Region that Modulates Tyrosine 926 Phosphorylation. Authors: Prutzman, K.C. / Gao, G. / King, M.L. / Iyer, V.V. / Mueller, G.A. / Schaller, M.D. / Campbell, S.L. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 877.2 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 735.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 346.5 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 540.9 KB | Display | |
Data in XML | ![]() | 76.2 KB | Display | |
Data in CIF | ![]() | 102.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-
Components
#1: Protein | Mass: 15897.431 Da / Num. of mol.: 1 / Fragment: Focal Adhesion Targeting (FAT) Domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
NMR experiment |
| ||||||||||||
NMR details | Text: PDB entry 1K40 was used as a starting template for structure calculations. The structure was determined using triple-resonance NMR spectroscopy. |
-
Sample preparation
Details | Contents: 0.85 mM Focal Adhesion Targeting Domain U-15N,13C, 25 mM Tris-Maleate, 0.1 % NaN3, 1.0 uM PPACK, 0.5 mg/mL Pefabloc 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
---|---|
Sample conditions | Ionic strength: 150 mM NaCl / pH: 6.0 / Pressure: ambient / Temperature: 310 K |
-NMR measurement
NMR spectrometer |
|
---|
-
Processing
NMR software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: distance geometry simulated annealing / Software ordinal: 1 Details: 3049 total restraints: 1627 unambiguous NOE-derived distance constraints, 1078 ambiguous NOE-derived distance constraints, 83 dihedral angle restraints, 97 distance restraints from hydrogen ...Details: 3049 total restraints: 1627 unambiguous NOE-derived distance constraints, 1078 ambiguous NOE-derived distance constraints, 83 dihedral angle restraints, 97 distance restraints from hydrogen bonds, 164 residual dipolar coupling restraints | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with acceptable covalent geometry,structures with the lowest energy Conformers calculated total number: 25 / Conformers submitted total number: 20 |