+Open data
-Basic information
Entry | Database: PDB / ID: 1pmd | ||||||
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Title | PENICILLIN-BINDING PROTEIN 2X (PBP-2X) | ||||||
Components | PEPTIDOGLYCAN SYNTHESIS MULTIFUNCTIONAL ENZYME | ||||||
Keywords | PEPTIDOGLYCAN SYNTHESIS / RESISTANCE / CELL WALL / TRANSMEMBRANE | ||||||
Function / homology | Function and homology information penicillin binding / peptidoglycan biosynthetic process / cell wall organization / regulation of cell shape / cell cycle / cell division / response to antibiotic / plasma membrane Similarity search - Function | ||||||
Biological species | Streptococcus pneumoniae (bacteria) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 3.5 Å | ||||||
Authors | Pares, S. / Mouz, N. / Dideberg, O. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1996 Title: X-ray structure of Streptococcus pneumoniae PBP2x, a primary penicillin target enzyme. Authors: Pares, S. / Mouz, N. / Petillot, Y. / Hakenbeck, R. / Dideberg, O. #1: Journal: J.Mol.Biol. / Year: 1993 Title: Crystallization of a Genetically Engineered Water-Soluble Primary Penicillin Target Enzyme. The High Molecular Mass Pbp2X of Streptococcus Pneumoniae Authors: Charlier, P. / Buisson, G. / Dideberg, O. / Wierenga, J. / Keck, W. / Laible, G. / Hakenbeck, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1pmd.cif.gz | 32.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1pmd.ent.gz | 15.3 KB | Display | PDB format |
PDBx/mmJSON format | 1pmd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pm/1pmd ftp://data.pdbj.org/pub/pdb/validation_reports/pm/1pmd | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 73919.656 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Streptococcus pneumoniae (bacteria) / References: UniProt: P14677 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 4.33 Å3/Da / Density % sol: 71.62 % | ||||||||||||||||||||||||||||||
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Crystal grow | *PLUS Temperature: 15 ℃ / pH: 4.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 3.5→30 Å / Num. obs: 16436 / % possible obs: 96.2 % / Observed criterion σ(I): 0 |
Reflection | *PLUS Rmerge(I) obs: 0.062 |
-Processing
Software |
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Refinement | Resolution: 3.5→6 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 3.5→6 Å
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Refine LS restraints |
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