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Open data
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Basic information
Entry | Database: PDB / ID: 1pl8 | ||||||
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Title | human SDH/NAD+ complex | ||||||
![]() | human sorbitol dehydrogenase | ||||||
![]() | OXIDOREDUCTASE / human sorbitol dehydrogenase / NAD | ||||||
Function / homology | ![]() ribitol 2-dehydrogenase / D-sorbitol dehydrogenase (acceptor) activity / ribitol 2-dehydrogenase (NAD+) activity / L-xylitol catabolic process / L-xylitol metabolic process / (R,R)-butanediol dehydrogenase / (R,R)-butanediol dehydrogenase activity / D-xylulose reductase / sorbitol catabolic process / D-xylulose reductase activity ...ribitol 2-dehydrogenase / D-sorbitol dehydrogenase (acceptor) activity / ribitol 2-dehydrogenase (NAD+) activity / L-xylitol catabolic process / L-xylitol metabolic process / (R,R)-butanediol dehydrogenase / (R,R)-butanediol dehydrogenase activity / D-xylulose reductase / sorbitol catabolic process / D-xylulose reductase activity / L-iditol 2-dehydrogenase / L-iditol 2-dehydrogenase (NAD+) activity / glucuronate catabolic process to xylulose 5-phosphate / Formation of xylulose-5-phosphate / flagellated sperm motility / Fructose biosynthesis / fructose biosynthetic process / Oxidoreductases; Acting on the CH-OH group of donors; With NAD+ or NADP+ as acceptor / motile cilium / mitochondrial membrane / glucose metabolic process / NAD binding / carbohydrate binding / extracellular space / zinc ion binding / extracellular exosome / identical protein binding / membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Pauly, T.A. / Ekstrom, J.L. / Beebe, D.A. / Chrunyk, B. / Cunningham, D. / Griffor, M. / Kamath, A. / Lee, S.E. / Madura, R. / Mcguire, D. ...Pauly, T.A. / Ekstrom, J.L. / Beebe, D.A. / Chrunyk, B. / Cunningham, D. / Griffor, M. / Kamath, A. / Lee, S.E. / Madura, R. / Mcguire, D. / Subashi, T. / Wasilko, D. / Watts, P. / Mylari, B.L. / Oates, P.J. / Adams, P.D. / Rath, V.L. | ||||||
![]() | ![]() Title: X-ray crystallographic and kinetic studies of human sorbitol dehydrogenase. Authors: Pauly, T.A. / Ekstrom, J.L. / Beebe, D.A. / Chrunyk, B. / Cunningham, D. / Griffor, M. / Kamath, A. / Lee, S.E. / Madura, R. / Mcguire, D. / Subashi, T. / Wasilko, D. / Watts, P. / Mylari, B. ...Authors: Pauly, T.A. / Ekstrom, J.L. / Beebe, D.A. / Chrunyk, B. / Cunningham, D. / Griffor, M. / Kamath, A. / Lee, S.E. / Madura, R. / Mcguire, D. / Subashi, T. / Wasilko, D. / Watts, P. / Mylari, B.L. / Oates, P.J. / Adams, P.D. / Rath, V.L. | ||||||
History |
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Remark 999 | The sequence variant Leu -> Gln for residue 238 is noted in Swiss-Prot entry Q00796. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 308.8 KB | Display | ![]() |
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PDB format | ![]() | 249.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 672.3 KB | Display | ![]() |
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Full document | ![]() | 693 KB | Display | |
Data in XML | ![]() | 31.7 KB | Display | |
Data in CIF | ![]() | 52.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 |
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Unit cell |
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Components
#1: Protein | Mass: 38229.332 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-NAD / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.79 Å3/Da / Density % sol: 67.57 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 6.15 Details: NH4AC, NaCitrate, PEG 4000, pH 6.15, VAPOR DIFFUSION, HANGING DROP, temperature 295K | ||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: BRANDEIS - B1.2 / Detector: CCD / Date: May 18, 2001 Details: double-crystal monochromator Si(111), beam focused by a toroidal mirror |
Radiation | Monochromator: double-crystal monochromator Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→99 Å / Num. obs: 161025 / % possible obs: 88.6 % / Observed criterion σ(I): 2 / Redundancy: 3.2 % / Biso Wilson estimate: 11.8 Å2 / Rsym value: 0.083 / Net I/σ(I): 12.8 |
Reflection shell | Resolution: 1.9→1.97 Å / Redundancy: 1 % / Rmerge(I) obs: 0.379 / Mean I/σ(I) obs: 2 / Num. unique all: 7993 / % possible all: 44.2 |
Reflection | *PLUS Highest resolution: 1.9 Å / Redundancy: 4 % / Rmerge(I) obs: 0.083 |
Reflection shell | *PLUS % possible obs: 44.2 % |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 54.2901 Å2 / ksol: 0.401316 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.2 Å2
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Refine analyze | Luzzati coordinate error free: 0.25 Å / Luzzati sigma a free: 0.24 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→45.82 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→2.02 Å / Rfactor Rfree error: 0.008 / Total num. of bins used: 6
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Xplor file |
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Refinement | *PLUS Highest resolution: 1.9 Å / Lowest resolution: 45.8 Å / % reflection Rfree: 10 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rwork: 0.27 |