Submitted confermers represent the 20 lowest energy structures with no NOE violations greater than 0.2 Angstroms and no dihedral violations greater than 5 degrees
代表モデル
モデル #1
lowest energy
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要素
#1: RNA鎖
HIV-1frameshiftinducingstem-loop
分子量: 7071.299 Da / 分子数: 1 / 由来タイプ: 合成 / 詳細: T7 RNA transcript from synthetic DNA
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実験情報
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実験
実験
手法: 溶液NMR
NMR実験
Conditions-ID
Experiment-ID
Solution-ID
タイプ
3
1
1
2D NOESY
3
2
1
2D TOCSY
3
3
1
3D 13C-separated NOESY
2
4
1
2D HNN-COSY
1
5
1
2D NOESY
3
6
1
2D 1H-13C HSQC
NMR実験の詳細
Text: The incorporation of residual dipolar couplings and the direct detection of hydrogen bonds with HNN-COSY were used in solving this structure
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試料調製
詳細
内容: 1 mM HIV-1 frameshift inducing stem-loop RNA / 溶媒系: 90% H2O/10% D2O, or 99.99% D2O
コンフォーマー選択の基準: Submitted confermers represent the 20 lowest energy structures with no NOE violations greater than 0.2 Angstroms and no dihedral violations greater than 5 degrees 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20