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Yorodumi- PDB-1bn0: SL3 HAIRPIN FROM THE PACKAGING SIGNAL OF HIV-1, NMR, 11 STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 1bn0 | ||||||
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Title | SL3 HAIRPIN FROM THE PACKAGING SIGNAL OF HIV-1, NMR, 11 STRUCTURES | ||||||
Components | SL3 RNA HAIRPIN | ||||||
Keywords | RNA / HIV-1 / PACKAGING / TETRALOOP / RIBONUCLEIC ACID | ||||||
Function / homology | RNA / RNA (> 10) Function and homology information | ||||||
Method | SOLUTION NMR / DISTANCE GEOMETRY, RESTRAINED MOLECULAR DYNAMICS | ||||||
Authors | Pappalardo, L. / Kerwood, D.J. / Pelczer, I. / Borer, P.N. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1998 Title: Three-dimensional folding of an RNA hairpin required for packaging HIV-1. Authors: Pappalardo, L. / Kerwood, D.J. / Pelczer, I. / Borer, P.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1bn0.cif.gz | 143.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1bn0.ent.gz | 116.6 KB | Display | PDB format |
PDBx/mmJSON format | 1bn0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1bn0_validation.pdf.gz | 328.2 KB | Display | wwPDB validaton report |
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Full document | 1bn0_full_validation.pdf.gz | 390.2 KB | Display | |
Data in XML | 1bn0_validation.xml.gz | 7 KB | Display | |
Data in CIF | 1bn0_validation.cif.gz | 10.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bn/1bn0 ftp://data.pdbj.org/pub/pdb/validation_reports/bn/1bn0 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 6477.919 Da / Num. of mol.: 1 / Source method: obtained synthetically |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: THE STRUCTURES WERE DETERMINED FROM 2D NOESY EXPERIMENTS PERFORMED BOTH IN H2O AND D2O. THE DISTANCE RESTRAINTS DETERMINED FROM MARDIGRAS WERE BASED ON INTENSITIES FROM A NOESY WITH A MIXING ...Text: THE STRUCTURES WERE DETERMINED FROM 2D NOESY EXPERIMENTS PERFORMED BOTH IN H2O AND D2O. THE DISTANCE RESTRAINTS DETERMINED FROM MARDIGRAS WERE BASED ON INTENSITIES FROM A NOESY WITH A MIXING TIME OF 300MS. DQF-COSY AND H-P COSY WERE USED TO ASCERTAIN INFORMATION ABOUT THE TORSION ANGLES. |
-Sample preparation
Details | Contents: WATER |
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Sample conditions | Ionic strength: 30mM / pH: 7 / Pressure: NORMAL / Temperature: 303 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Bruker DRX500 / Manufacturer: Bruker / Model: DRX500 / Field strength: 500 MHz |
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-Processing
Software | Name: AMBER / Classification: refinement | |||||||||||||||
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NMR software |
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Refinement | Method: DISTANCE GEOMETRY, RESTRAINED MOLECULAR DYNAMICS / Software ordinal: 1 Details: AVERAGE SIXTH ROOT RESIDUAL NMR INDEX = 0.07, AVERAGE RESTRAINT VIOLATION = 0.06 ANGSTROM. | |||||||||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 500 / Conformers submitted total number: 11 |