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- PDB-1pie: Crystal Structure of Lactococcus lactis Galactokinase Complexed w... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1pie | ||||||
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Title | Crystal Structure of Lactococcus lactis Galactokinase Complexed with Galactose | ||||||
![]() | Galactokinase | ||||||
![]() | TRANSFERASE / galactose / galactosemia / kinase | ||||||
Function / homology | ![]() galactokinase / galactokinase activity / galactose metabolic process / magnesium ion binding / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Thoden, J.B. / Holden, H.M. | ||||||
![]() | ![]() Title: Molecular Structure of Galactokinase Authors: Thoden, J.B. / Holden, H.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 91.5 KB | Display | ![]() |
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PDB format | ![]() | 68.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 463 KB | Display | ![]() |
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Full document | ![]() | 489.1 KB | Display | |
Data in XML | ![]() | 21 KB | Display | |
Data in CIF | ![]() | 29.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 46084.941 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: GALK / Plasmid: pET28 / Production host: ![]() ![]() |
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#2: Sugar | ChemComp-GLA / |
#3: Chemical | ChemComp-PO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.67 Å3/Da / Density % sol: 53.86 % | ||||||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: batch / pH: 9 Details: sodium phosphate, potassium phosphate, CHES, galactose, pH 9, batch, temperature 277K | ||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: microdialysis | ||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
Detector | Type: SBC-2 / Detector: CCD / Date: Apr 4, 2003 | |||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Resolution: 2.1→20 Å / Num. all: 28616 / Num. obs: 28616 / % possible obs: 98.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 14 % / Rsym value: 0.067 / Net I/σ(I): 75.4 | |||||||||||||||
Reflection shell | Resolution: 2.1→2.17 Å / Redundancy: 9.7 % / Mean I/σ(I) obs: 9.3 / Num. unique all: 2804 / Rsym value: 0.214 / % possible all: 99.5 | |||||||||||||||
Reflection | *PLUS Rmerge(I) obs: 0.067 | |||||||||||||||
Reflection shell | *PLUS % possible obs: 99.5 % / Num. unique obs: 2804 / Rmerge(I) obs: 0.214 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 2.1→20 Å
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Refine LS restraints |
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Refinement | *PLUS | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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