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- PDB-1pfm: PF4-M2 CHIMERIC MUTANT WITH THE FIRST 10 N-TERMINAL RESIDUES OF R... -

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Basic information

Entry
Database: PDB / ID: 1pfm
TitlePF4-M2 CHIMERIC MUTANT WITH THE FIRST 10 N-TERMINAL RESIDUES OF R-PF4 REPLACED BY THE N-TERMINAL RESIDUES OF THE IL8 SEQUENCE. MODELS 1-15 OF A 27-MODEL SET.
ComponentsPF4-M2 CHIMERA
KeywordsCYTOKINE
Function / homology
Function and homology information


CXCR3 chemokine receptor binding / CXCR chemokine receptor binding / negative regulation of MHC class II biosynthetic process / killing by host of symbiont cells / positive regulation of macrophage differentiation / positive regulation of macrophage derived foam cell differentiation / leukocyte chemotaxis / chemokine activity / Chemokine receptors bind chemokines / defense response to protozoan ...CXCR3 chemokine receptor binding / CXCR chemokine receptor binding / negative regulation of MHC class II biosynthetic process / killing by host of symbiont cells / positive regulation of macrophage differentiation / positive regulation of macrophage derived foam cell differentiation / leukocyte chemotaxis / chemokine activity / Chemokine receptors bind chemokines / defense response to protozoan / monocyte chemotaxis / negative regulation of extrinsic apoptotic signaling pathway in absence of ligand / negative regulation of blood coagulation / negative regulation of megakaryocyte differentiation / Common Pathway of Fibrin Clot Formation / neutrophil chemotaxis / negative regulation of angiogenesis / platelet alpha granule lumen / Cell surface interactions at the vascular wall / RUNX1 regulates genes involved in megakaryocyte differentiation and platelet function / cytokine-mediated signaling pathway / platelet activation / adenylate cyclase-activating G protein-coupled receptor signaling pathway / antimicrobial humoral immune response mediated by antimicrobial peptide / positive regulation of tumor necrosis factor production / Platelet degranulation / heparin binding / regulation of cell population proliferation / cellular response to lipopolysaccharide / : / G alpha (i) signalling events / inflammatory response / receptor ligand activity / positive regulation of gene expression / positive regulation of transcription by RNA polymerase II / extracellular space / extracellular region / cytoplasm
Similarity search - Function
CXC chemokine / CXC chemokine, conserved site / Small cytokines (intercrine/chemokine) C-x-C subfamily signature. / CXC Chemokine domain / Chemokine beta/gamma/delta / Intercrine alpha family (small cytokine C-X-C) (chemokine CXC). / Chemokine interleukin-8-like domain / Chemokine interleukin-8-like superfamily / Small cytokines (intecrine/chemokine), interleukin-8 like / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #40 ...CXC chemokine / CXC chemokine, conserved site / Small cytokines (intercrine/chemokine) C-x-C subfamily signature. / CXC Chemokine domain / Chemokine beta/gamma/delta / Intercrine alpha family (small cytokine C-X-C) (chemokine CXC). / Chemokine interleukin-8-like domain / Chemokine interleukin-8-like superfamily / Small cytokines (intecrine/chemokine), interleukin-8 like / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #40 / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Beta Barrel / Mainly Beta
Similarity search - Domain/homology
Biological speciesHomo sapiens (human)
MethodSOLUTION NMR
AuthorsMayo, K.H. / Roongta, V. / Ilyina, E. / Milius, R. / Barker, S. / Quinlan, C. / La Rosa, G. / Daly, T.J.
Citation
Journal: Biochemistry / Year: 1995
Title: NMR solution structure of the 32-kDa platelet factor 4 ELR-motif N-terminal chimera: a symmetric tetramer.
Authors: Mayo, K.H. / Roongta, V. / Ilyina, E. / Milius, R. / Barker, S. / Quinlan, C. / La Rosa, G. / Daly, T.J.
#1: Journal: Biochemistry / Year: 1994
Title: Crystal Structure of Recombinant Human Platelet Factor 4
Authors: Zhang, X. / Chen, L. / Bancroft, D.P. / Lai, C.K. / Maione, T.E.
History
DepositionJul 18, 1995Processing site: BNL
Revision 1.0Jan 29, 1996Provider: repository / Type: Initial release
Revision 1.1Mar 24, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Nov 3, 2021Group: Database references / Derived calculations / Other
Category: database_2 / pdbx_database_status ...database_2 / pdbx_database_status / pdbx_struct_assembly / pdbx_struct_oper_list / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.process_site / _struct_ref_seq_dif.details
Revision 1.4Nov 20, 2024Group: Data collection / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_entry_details / pdbx_modification_feature

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: PF4-M2 CHIMERA
B: PF4-M2 CHIMERA
C: PF4-M2 CHIMERA
D: PF4-M2 CHIMERA


Theoretical massNumber of molelcules
Total (without water)30,5134
Polymers30,5134
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)15 / -
Representative

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Components

#1: Protein
PF4-M2 CHIMERA / PLATELET FACTOR 4 / PLATELET FACTOR M2


Mass: 7628.229 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Cell line: BL21 / Plasmid: BL21 / Production host: Escherichia coli (E. coli) / References: UniProt: P02776
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR

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Sample preparation

Crystal grow
*PLUS
Method: other

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Processing

Software
NameClassification
X-PLORmodel building
X-PLORrefinement
X-PLORphasing
NMR softwareName: X-PLOR / Developer: BRUNGER / Classification: refinement
NMR ensembleConformers submitted total number: 15

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