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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 1pfk | ||||||
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タイトル | CRYSTAL STRUCTURE OF THE COMPLEX OF PHOSPHOFRUCTOKINASE FROM ESCHERICHIA COLI WITH ITS REACTION PRODUCTS | ||||||
![]() | PHOSPHOFRUCTOKINASE | ||||||
![]() | TRANSFERASE(PHOSPHOTRANSFERASE) | ||||||
機能・相同性 | ![]() 6-phosphofructokinase complex / 6-phosphofructokinase / 6-phosphofructokinase activity / ribonucleotide binding / fructose-6-phosphate binding / glucose catabolic process / fructose 6-phosphate metabolic process / canonical glycolysis / monosaccharide binding / fructose 1,6-bisphosphate metabolic process ...6-phosphofructokinase complex / 6-phosphofructokinase / 6-phosphofructokinase activity / ribonucleotide binding / fructose-6-phosphate binding / glucose catabolic process / fructose 6-phosphate metabolic process / canonical glycolysis / monosaccharide binding / fructose 1,6-bisphosphate metabolic process / AMP binding / glycolytic process / GDP binding / protein homotetramerization / magnesium ion binding / ATP binding / identical protein binding / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | ![]() ![]() | ||||||
手法 | ![]() | ||||||
![]() | Shirakihara, Y. / Evans, P.R. | ||||||
![]() | ![]() タイトル: Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products. 著者: Shirakihara, Y. / Evans, P.R. #1: ![]() タイトル: Crystal Structure of Unliganded Phosphofructokinase from Escherichia Coli 著者: Rypniewski, W.R. / Evans, P.R. #2: ![]() タイトル: Crystallographic Structure of Allosterically Inhibited Phosphofructokinase at 7 Angstroms Resolution 著者: Evans, P.R. / Farrants, G.W. / Lawrence, M.C. #3: ![]() タイトル: Nucleotide Sequence and High-Level Expression of the Major Escherichia Coli Phosphofructokinase 著者: Hellinga, H.W. / Evans, P.R. #4: ![]() タイトル: Phosphofructokinase. Structure and Control 著者: Evans, P.R. / Farrants, G.W. / Hudson, P.J. #5: ![]() タイトル: Structure and Control of Phosphofructokinase from Bacillus Stearothermophilus 著者: Evans, P.R. / Hudson, P.J. #6: ![]() タイトル: The Three-Dimensional Structure of Phosphofructokinase from Bacillus Stearothermophilus 著者: Evans, P.R. / Hudson, P.J. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 143.8 KB | 表示 | ![]() |
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PDB形式 | ![]() | 112.5 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
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-関連構造データ
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper: (Code: given / Matrix: (0.54287, 0.83982),詳細 | THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT CONTAINS TWO SUBUNITS OF THE TETRAMER. THESE SUBUNITS HAVE BEEN ASSIGNED CHAIN IDENTIFIERS *A* AND *B* AND HAVE DIFFERENT CONFORMATIONS. CHAIN A IS A *CLOSED* SUBUNIT WITH THE MG++ ION BRIDGING THE TWO PRODUCTS. CHAIN B IS AN *OPEN* SUBUNIT WITH THE MG++ BOUND TO ADP ONLY. THERE ARE TWO WATER CHAINS, ONE CORRESPONDING TO EACH SUBUNIT, AND EQUIVALENT WATER MOLECULES IN EACH CHAIN HAVE BEEN ASSIGNED THE SAME RESIDUE NUMBER. THE TETRAMER CAN BE COMPLETED FROM THE DIMER IN THIS ENTRY BY ROTATING 180 DEGREES ABOUT Z (I.E. -X, -Y, Z). THE STORED ORTHOGONAL CRYSTALLOGRAPHIC COORDINATES IN THIS ENTRY MAY BE CONVERTED TO THE MOLECULAR PQR FRAME (WITH THE MOLECULAR DIADS ALONG P,Q,R) BY THE TRANSFORMATION 0.87831 0.47808 0.00000 0.00000 0.00000 0.00000 -1.00000 -2.71300 -0.47808 0.87831 0.00000 0.00000 THE TRANSFORMATION SPECIFIED ON THE *MTRIX* RECORDS BELOW WILL YIELD APPROXIMATE COORDINATES FOR THE B CHAIN WHEN APPLIED TO THE A CHAIN. IT WILL ALSO YIELD APPROXIMATE COORDINATES FOR THE A CHAIN WHEN APPLIED TO THE B CHAIN. | |
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要素
#1: タンパク質 | 分子量: 34885.047 Da / 分子数: 2 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() #2: 糖 | #3: 化合物 | ChemComp-MG / #4: 化合物 | ChemComp-ADP / #5: 水 | ChemComp-HOH / | 非ポリマーの詳細 | ADP A 324 AND ADP B 324 ARE THE PRODUCTS OF THE CATALYTIC REACTION ATP + F6P --> ADP + F-1,6-DP. ...ADP A 324 AND ADP B 324 ARE THE PRODUCTS OF THE CATALYTIC REACTION ATP + F6P --> ADP + F-1,6-DP. ADP A 326 AND ADP B 326 ARE THE ALLOSTERIC | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.65 Å3/Da / 溶媒含有率: 53.56 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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結晶化 | *PLUS 手法: batch method / pH: 8.3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶液の組成 | *PLUS
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-データ収集
放射 | 散乱光タイプ: x-ray |
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放射波長 | 相対比: 1 |
反射 | *PLUS 最高解像度: 2.4 Å / 最低解像度: 6 Å / Num. obs: 27155 / % possible obs: 74 % / Num. measured all: 93331 / Rmerge(I) obs: 0.133 |
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解析
ソフトウェア | 名称: PROLSQ / 分類: 精密化 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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精密化 | 解像度: 2.4→100 Å / Rfactor obs: 0.165 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化ステップ | サイクル: LAST / 解像度: 2.4→100 Å
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拘束条件 |
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ソフトウェア | *PLUS 名称: PROLSQ / 分類: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
精密化 | *PLUS Num. reflection obs: 27127 / Rfactor obs: 0.165 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
溶媒の処理 | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
拘束条件 | *PLUS
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