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- PDB-1p9i: Coiled-coil X-ray structure at 1.17 A resolution -

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Basic information

Entry
Database: PDB / ID: 1p9i
TitleCoiled-coil X-ray structure at 1.17 A resolution
Componentscortexillin I/GCN4 hybrid peptide
KeywordsUNKNOWN FUNCTION / coiled-coil
MethodX-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.17 Å
AuthorsIvaninskii, S.
CitationJournal: Protein Sci. / Year: 2003
Title: Unique stabilizing interactions identified in the two-stranded alpha-helical coiled-coil: crystal structure of a cortexillin I/GCN4 hybrid coiled-coil peptide.
Authors: Lee, D.L. / Ivaninskii, S. / Burkhard, P. / Hodges, R.S.
History
DepositionMay 12, 2003Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 23, 2003Provider: repository / Type: Initial release
Revision 1.1Apr 29, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 14, 2024Group: Data collection / Database references / Category: chem_comp_atom / chem_comp_bond / database_2
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Remark 999This peptide is a hybrid of two proteins: GCN4 and cortexillin I.

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: cortexillin I/GCN4 hybrid peptide


Theoretical massNumber of molelcules
Total (without water)3,4011
Polymers3,4011
Non-polymers00
Water57632
1
A: cortexillin I/GCN4 hybrid peptide

A: cortexillin I/GCN4 hybrid peptide


Theoretical massNumber of molelcules
Total (without water)6,8022
Polymers6,8022
Non-polymers00
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation3_655-x+1,y,-z+1/21
Unit cell
Length a, b, c (Å)18.098, 117.287, 22.209
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number20
Space group name H-MC2221
Components on special symmetry positions
IDModelComponents
11A-1017-

HOH

21A-1020-

HOH

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Components

#1: Protein/peptide cortexillin I/GCN4 hybrid peptide


Mass: 3400.935 Da / Num. of mol.: 1 / Source method: obtained synthetically
#2: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 32 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 1.73 Å3/Da / Density % sol: 29.01 %
Crystal grow
*PLUS
pH: 4.6 / Method: vapor diffusion, hanging drop
Components of the solutions
*PLUS
IDConc.Common nameCrystal-IDSol-IDDetails
10.1-0.2 Mammonium acetate1reservoir
230 %PEG40001reservoir
30.1 Msodium acetate1reservoirpH4.6
420 mg/mlprotein1drop

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Data collection

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthRelative weight: 1
ReflectionResolution: 1.17→20.77 Å / Num. all: 8174 / Num. obs: 8174
Reflection
*PLUS
Num. obs: 8183 / % possible obs: 97.8 % / Num. measured all: 263880 / Rmerge(I) obs: 0.037

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Processing

RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.17→20 Å /
RfactorNum. reflection
Rfree0.217 -
Rwork0.179 -
obs-8174
Refinement stepCycle: LAST / Resolution: 1.17→20 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms225 0 0 32 257
Refine LS restraints
*PLUS
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONbond_d0.009
X-RAY DIFFRACTIONangle_d
X-RAY DIFFRACTIONangle_deg1.8

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