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Yorodumi- PDB-1p8d: X-Ray Crystal Structure of LXR Ligand Binding Domain with 24(S),2... -
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-Basic information
Entry | Database: PDB / ID: 1p8d | ||||||
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Title | X-Ray Crystal Structure of LXR Ligand Binding Domain with 24(S),25-epoxycholesterol | ||||||
Components |
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Keywords | MEMBRANE PROTEIN/PROTEIN BINDING / LXR / epoxycholesterol / nuclear receptor / steroid receptor / liver X receptor / transcription / MEMBRANE PROTEIN-PROTEIN BINDING COMPLEX | ||||||
Function / homology | Function and homology information positive regulation of secretion of lysosomal enzymes / positive regulation of high-density lipoprotein particle assembly / positive regulation of pancreatic juice secretion / phosphatidylcholine acyl-chain remodeling / positive regulation of cholesterol transport / negative regulation of response to endoplasmic reticulum stress / negative regulation of pinocytosis / positive regulation of lipoprotein lipase activity / apolipoprotein A-I receptor binding / positive regulation of triglyceride biosynthetic process ...positive regulation of secretion of lysosomal enzymes / positive regulation of high-density lipoprotein particle assembly / positive regulation of pancreatic juice secretion / phosphatidylcholine acyl-chain remodeling / positive regulation of cholesterol transport / negative regulation of response to endoplasmic reticulum stress / negative regulation of pinocytosis / positive regulation of lipoprotein lipase activity / apolipoprotein A-I receptor binding / positive regulation of triglyceride biosynthetic process / NR1H2 & NR1H3 regulate gene expression linked to triglyceride lipolysis in adipose / NR1H2 & NR1H3 regulate gene expression to limit cholesterol uptake / positive regulation of lipid storage / NR1H2 & NR1H3 regulate gene expression linked to lipogenesis / positive regulation of fatty acid biosynthetic process / negative regulation of lipid transport / labyrinthine layer morphogenesis / regulation of thyroid hormone mediated signaling pathway / positive regulation of transcription from RNA polymerase II promoter by galactose / positive regulation of female receptivity / negative regulation of cold-induced thermogenesis / hypothalamus development / male mating behavior / NR1H2 & NR1H3 regulate gene expression to control bile acid homeostasis / negative regulation of type II interferon-mediated signaling pathway / negative regulation of cholesterol storage / estrous cycle / positive regulation of cholesterol efflux / negative regulation of macrophage derived foam cell differentiation / cellular response to Thyroglobulin triiodothyronine / Synthesis of bile acids and bile salts / Endogenous sterols / Synthesis of bile acids and bile salts via 27-hydroxycholesterol / Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol / nuclear retinoid X receptor binding / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / response to retinoic acid / histone acetyltransferase activity / Recycling of bile acids and salts / regulation of cellular response to insulin stimulus / histone acetyltransferase / cellular response to hormone stimulus / positive regulation of adipose tissue development / peroxisome proliferator activated receptor signaling pathway / positive regulation of neuron differentiation / RORA activates gene expression / VLDLR internalisation and degradation / positive regulation of protein metabolic process / lactation / Regulation of lipid metabolism by PPARalpha / hormone-mediated signaling pathway / cerebellum development / cholesterol homeostasis / BMAL1:CLOCK,NPAS2 activates circadian gene expression / SUMOylation of transcription cofactors / nuclear receptor coactivator activity / Activation of gene expression by SREBF (SREBP) / response to progesterone / nuclear estrogen receptor binding / nuclear receptor binding / hippocampus development / RNA polymerase II transcription regulatory region sequence-specific DNA binding / negative regulation of proteolysis / SUMOylation of intracellular receptors / Heme signaling / mRNA transcription by RNA polymerase II / Transcriptional activation of mitochondrial biogenesis / PPARA activates gene expression / Cytoprotection by HMOX1 / cerebral cortex development / chromatin DNA binding / Transcriptional regulation of white adipocyte differentiation / positive regulation of miRNA transcription / Nuclear Receptor transcription pathway / RNA polymerase II transcription regulator complex / male gonad development / nuclear receptor activity / Circadian Clock / response to estradiol / HATs acetylate histones / ATPase binding / DNA-binding transcription activator activity, RNA polymerase II-specific / Estrogen-dependent gene expression / transcription regulator complex / cell differentiation / transcription coactivator activity / protein dimerization activity / DNA-binding transcription factor activity, RNA polymerase II-specific / positive regulation of apoptotic process / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of DNA-templated transcription / chromatin binding / chromatin / positive regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / zinc ion binding / nucleoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Williams, S. / Bledsoe, R.K. / Collins, J.L. / Boggs, S. / Lambert, M.H. / Miller, A.B. / Moore, J. / McKee, D.D. / Moore, L. / Nichols, J. ...Williams, S. / Bledsoe, R.K. / Collins, J.L. / Boggs, S. / Lambert, M.H. / Miller, A.B. / Moore, J. / McKee, D.D. / Moore, L. / Nichols, J. / Parks, D. / Watson, M. / Wisely, B. / Willson, T.M. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2003 Title: X-ray crystal structure of the liver X receptor beta ligand binding domain: regulation by a histidine-tryptophan switch. Authors: Williams, S. / Bledsoe, R.K. / Collins, J.L. / Boggs, S. / Lambert, M.H. / Miller, A.B. / Moore, J. / McKee, D.D. / Moore, L. / Nichols, J. / Parks, D. / Watson, M. / Wisely, B. / Willson, T.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1p8d.cif.gz | 117.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1p8d.ent.gz | 92.7 KB | Display | PDB format |
PDBx/mmJSON format | 1p8d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p8/1p8d ftp://data.pdbj.org/pub/pdb/validation_reports/p8/1p8d | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 28730.887 Da / Num. of mol.: 2 Fragment: Liver X Receptor beta ligand binding domain (residues 214-461) Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: NR1H2 OR LXRB OR UNR OR NER / Plasmid: pHTC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21[DE3] / References: UniProt: P55055 #2: Protein/peptide | Mass: 2806.163 Da / Num. of mol.: 2 Fragment: Steroid Receptor Co-activator 1 (residues 676-700) Source method: obtained synthetically / Details: Pepetide synthesis / References: GenBank: 22538459, UniProt: Q15788*PLUS #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.73 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 10-12% PEG3350-8000, 0.2M NaCl , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 17-ID / Wavelength: 1 Å |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Dec 14, 2001 / Details: monochromator |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→20 Å / Num. all: 15890 / Num. obs: 15658 / % possible obs: 98.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3 % / Biso Wilson estimate: 19 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 12.9 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 3 % / Rmerge(I) obs: 0.36 / Mean I/σ(I) obs: 3 / Num. unique all: 2227 / % possible all: 99.3 |
Reflection | *PLUS Highest resolution: 2.7 Å / Num. obs: 19175 / % possible obs: 95.3 % / Num. measured all: 141128 / Rmerge(I) obs: 0.055 |
Reflection shell | *PLUS % possible obs: 81.6 % / Rmerge(I) obs: 0.28 / Mean I/σ(I) obs: 4.8 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: RXR LBD Resolution: 2.8→20 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
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Refine LS restraints |
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Refinement | *PLUS Highest resolution: 2.7 Å / % reflection Rfree: 7 % / Rfactor Rfree: 0.276 / Rfactor Rwork: 0.215 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: c_bond_d / Dev ideal: 0.014 |