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Yorodumi- PDB-1p4q: Solution structure of the CITED2 transactivation domain in comple... -
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-Basic information
Entry | Database: PDB / ID: 1p4q | ||||||
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Title | Solution structure of the CITED2 transactivation domain in complex with the p300 CH1 domain | ||||||
Components |
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Keywords | TRANSCRIPTION/TRANSFERASE / helix / protein-protein complex / TRANSCRIPTION-TRANSFERASE COMPLEX | ||||||
Function / homology | Function and homology information cranial nerve morphogenesis / TFAP2 (AP-2) family regulates transcription of other transcription factors / embryonic process involved in female pregnancy / left/right pattern formation / pulmonary artery morphogenesis / embryonic heart tube left/right pattern formation / regulation of animal organ formation / adrenal cortex formation / nodal signaling pathway / embryonic camera-type eye morphogenesis ...cranial nerve morphogenesis / TFAP2 (AP-2) family regulates transcription of other transcription factors / embryonic process involved in female pregnancy / left/right pattern formation / pulmonary artery morphogenesis / embryonic heart tube left/right pattern formation / regulation of animal organ formation / adrenal cortex formation / nodal signaling pathway / embryonic camera-type eye morphogenesis / sex determination / positive regulation of male gonad development / behavioral defense response / endocardial cushion development / protein propionyltransferase activity / peptidyl-lysine propionylation / histone lactyltransferase activity / peptidyl-lysine crotonylation / peptidyl-lysine butyrylation / histone butyryltransferase activity / histone H3K122 acetyltransferase activity / swimming / peptide butyryltransferase activity / determination of left/right asymmetry in lateral mesoderm / histone H2B acetyltransferase activity / thigmotaxis / positive regulation of peroxisome proliferator activated receptor signaling pathway / granulocyte differentiation / peptide 2-hydroxyisobutyryltransferase activity / histone crotonyltransferase activity / lens morphogenesis in camera-type eye / left/right axis specification / NOTCH2 intracellular domain regulates transcription / positive regulation of cell-cell adhesion / lysine N-acetyltransferase activity, acting on acetyl phosphate as donor / negative regulation of cardiac muscle cell proliferation / peptidyl-lysine acetylation / response to fluid shear stress / histone H3 acetyltransferase activity / histone H4 acetyltransferase activity / trophectodermal cell differentiation / cellular response to L-leucine / internal peptidyl-lysine acetylation / peripheral nervous system development / cardiac neural crest cell development involved in heart development / NFE2L2 regulating ER-stress associated genes / peptide N-acetyltransferase activity / STAT3 nuclear events downstream of ALK signaling / acetylation-dependent protein binding / Activation of the TFAP2 (AP-2) family of transcription factors / NFE2L2 regulating inflammation associated genes / NGF-stimulated transcription / histone H3K27 acetyltransferase activity / histone H3K18 acetyltransferase activity / Polo-like kinase mediated events / N-terminal peptidyl-lysine acetylation / LRR FLII-interacting protein 1 (LRRFIP1) activates type I IFN production / NFE2L2 regulates pentose phosphate pathway genes / regulation of androgen receptor signaling pathway / NFE2L2 regulating MDR associated enzymes / positive regulation by host of viral transcription / bone morphogenesis / regulation of mitochondrion organization / determination of left/right symmetry / face morphogenesis / Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells / RUNX3 regulates NOTCH signaling / NOTCH4 Intracellular Domain Regulates Transcription / Regulation of FOXO transcriptional activity by acetylation / Regulation of gene expression by Hypoxia-inducible Factor / Nuclear events mediated by NFE2L2 / Regulation of NFE2L2 gene expression / NOTCH3 Intracellular Domain Regulates Transcription / regulation of glycolytic process / TRAF6 mediated IRF7 activation / platelet formation / histone acetyltransferase binding / megakaryocyte development / peptide-lysine-N-acetyltransferase activity / erythrocyte development / nuclear androgen receptor binding / NFE2L2 regulating anti-oxidant/detoxification enzymes / regulation of tubulin deacetylation / macrophage derived foam cell differentiation / FOXO-mediated transcription of cell death genes / NFE2L2 regulating tumorigenic genes / internal protein amino acid acetylation / STAT family protein binding / acyltransferase activity / ventricular septum morphogenesis / LBD domain binding / heart looping / protein acetylation / fat cell differentiation / Formation of paraxial mesoderm / RUNX1 interacts with co-factors whose precise effect on RUNX1 targets is not known / outflow tract morphogenesis / positive regulation of transforming growth factor beta receptor signaling pathway / SMAD binding / skeletal muscle cell differentiation Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Freedman, S.J. / Sun, Z.-Y.J. / Kung, A.L. / France, D.S. / Wagner, G. / Eck, M.J. | ||||||
Citation | Journal: Nat.Struct.Biol. / Year: 2003 Title: Structural basis for negative regulation of hypoxia-inducible factor-1alpha by CITED2. Authors: Freedman, S.J. / Sun, Z.Y. / Kung, A.L. / France, D.S. / Wagner, G. / Eck, M.J. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 2002 Title: Structural basis for recruitment of CBP/p300 by hypoxia-inducible factor-1alpha | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1p4q.cif.gz | 784.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1p4q.ent.gz | 677.9 KB | Display | PDB format |
PDBx/mmJSON format | 1p4q.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p4/1p4q ftp://data.pdbj.org/pub/pdb/validation_reports/p4/1p4q | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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NMR ensembles |
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-Components
#1: Protein | Mass: 5800.417 Da / Num. of mol.: 1 / Fragment: transactivation domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: CITED2 OR MRG1 / Plasmid: pET / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q99967 |
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#2: Protein | Mass: 11425.289 Da / Num. of mol.: 1 / Fragment: cysteine/histidine-rich 1 (CH1) domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: EP300 OR P300 / Plasmid: pACYC / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q09472, histone acetyltransferase |
#3: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 100mM NaCl / pH: 6 / Pressure: ambient / Temperature: 298 K | |||||||||||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 25 / Conformers submitted total number: 17 |