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- PDB-1owc: Three Dimensional Structure Analysis Of The R109L Variant of the ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1owc | ||||||
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Title | Three Dimensional Structure Analysis Of The R109L Variant of the Type II Citrate Synthase From E. Coli | ||||||
![]() | Citrate synthase | ||||||
![]() | TRANSFERASE / Allostery / NADH / Type II Citrate Synthase / E. Coli / R109L | ||||||
Function / homology | ![]() citrate synthase (unknown stereospecificity) / citrate (Si)-synthase activity / NADH binding / protein hexamerization / tricarboxylic acid cycle / identical protein binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Stokell, D.J. / Donald, L.J. / Maurus, R. / Nguyen, N.T. / Sadler, G. / Choudhary, K. / Hultin, P.G. / Brayer, G.D. / Duckworth, H.W. | ||||||
![]() | ![]() Title: Probing the roles of key residues in the unique regulatory NADH binding site of type II citrate synthase of Escherichia coli. Authors: Stokell, D.J. / Donald, L.J. / Maurus, R. / Nguyen, N.T. / Sadler, G. / Choudhary, K. / Hultin, P.G. / Brayer, G.D. / Duckworth, H.W. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 185.9 KB | Display | ![]() |
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PDB format | ![]() | 147.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 456 KB | Display | ![]() |
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Full document | ![]() | 479.2 KB | Display | |
Data in XML | ![]() | 38.5 KB | Display | |
Data in CIF | ![]() | 55.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1owbC ![]() 1k3p C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 48080.957 Da / Num. of mol.: 2 / Mutation: R109L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.23 Å3/Da / Density % sol: 70.94 % |
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Crystal grow | Method: vapor diffusion, hanging drop / Details: VAPOR DIFFUSION, HANGING DROP |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IIC / Detector: IMAGE PLATE / Date: Jul 3, 2000 / Details: osmic mirror |
Radiation | Monochromator: Osmic Mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 2.2 Å / Num. obs: 72746 / % possible obs: 88 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.6 % / Rmerge(I) obs: 0.094 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1K3P ![]() 1k3p Resolution: 2.2→10 Å / Stereochemistry target values: Engh & Huber /
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Refinement step | Cycle: LAST / Resolution: 2.2→10 Å
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