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Yorodumi- PDB-1ovy: Solution Structure of Ribosomal Protein L18 from Bacillus stearot... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1ovy | ||||||
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Title | Solution Structure of Ribosomal Protein L18 from Bacillus stearothermophilus | ||||||
Components | 50S Ribosomal Protein L18 | ||||||
Keywords | RIBOSOME / Ribosomal Protein | ||||||
Function / homology | Function and homology information rRNA binding / ribosome / structural constituent of ribosome / translation Similarity search - Function | ||||||
Biological species | Geobacillus stearothermophilus (bacteria) | ||||||
Method | SOLUTION NMR / Aria was used to select, assign peak lists from NOESY experiments. CNS as modified for use with ARIA was used for structure calculation using torsion angle simulated annealing. | ||||||
Authors | Turner, C.F. / Moore, P.B. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: The solution structure of ribosomal protein L18 from Bacillus stearothermophilus Authors: Turner, C.F. / Moore, P.B. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1ovy.cif.gz | 336.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1ovy.ent.gz | 281.1 KB | Display | PDB format |
PDBx/mmJSON format | 1ovy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1ovy_validation.pdf.gz | 349.7 KB | Display | wwPDB validaton report |
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Full document | 1ovy_full_validation.pdf.gz | 504.5 KB | Display | |
Data in XML | 1ovy_validation.xml.gz | 36.6 KB | Display | |
Data in CIF | 1ovy_validation.cif.gz | 49.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ov/1ovy ftp://data.pdbj.org/pub/pdb/validation_reports/ov/1ovy | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13500.638 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Geobacillus stearothermophilus (bacteria) Gene: RPLR / Plasmid: pET13a / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P09415 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using triple resonance NMR spectroscopy. |
-Sample preparation
Details |
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Sample conditions | Ionic strength: 12mM KH2PO4; 8mM NaH2PO4; 200mM NaCl, 5mM MgCl2 pH: 6.0 / Pressure: 1 atm / Temperature: 298 K | ||||||||||||
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: Aria was used to select, assign peak lists from NOESY experiments. CNS as modified for use with ARIA was used for structure calculation using torsion angle simulated annealing. Software ordinal: 1 Details: The structures are based on a total of 2272 restraints. 1188 are unambiguous distance constraints, 913 are ambiguous distance constraints, 128 are dihedral angle restraints, and 43 are hydrogen bond restraints. | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 150 / Conformers submitted total number: 10 |