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- PDB-1or6: Crystal Structure of HemAT sensor domain from B.subtilis in the u... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1or6 | ||||||
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Title | Crystal Structure of HemAT sensor domain from B.subtilis in the unliganded form | ||||||
![]() | Heme-based aerotactic transducer hemAT | ||||||
![]() | SIGNALING PROTEIN / Globin fold | ||||||
Function / homology | ![]() aerotaxis / oxygen binding / heme binding / signal transduction / membrane / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, W. / Phillips Jr., G.N. | ||||||
![]() | ![]() Title: Structure of the oxygen sensor in Bacillus subtilis: signal transduction of chemotaxis by control of symmetry. Authors: Zhang, W. / Phillips, G.N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 84.8 KB | Display | ![]() |
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PDB format | ![]() | 63.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 18.4 KB | Display | |
Data in CIF | ![]() | 25.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1or4SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20841.094 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.01 Å3/Da / Density % sol: 38.9 % | ||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 Details: sodium citrate, KH2PO4, n-Octyl-D-glucoside , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K | ||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 5, 2002 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.71→30 Å / Num. all: 15592 / Num. obs: 15592 / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 / Biso Wilson estimate: 15 Å2 / Rmerge(I) obs: 0.098 |
Reflection shell | Resolution: 2.7→2.8 Å / Rmerge(I) obs: 0.216 / % possible all: 74 |
Reflection | *PLUS Lowest resolution: 27 Å / Num. obs: 9226 / % possible obs: 95.1 % / Num. measured all: 55718 |
Reflection shell | *PLUS % possible obs: 74 % / Mean I/σ(I) obs: 6.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1OR4 Resolution: 2.71→26.74 Å / Rfactor Rfree error: 0.009 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 58.9327 Å2 / ksol: 0.375785 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.1 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.71→26.74 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.71→2.81 Å / Rfactor Rfree error: 0.045 / Total num. of bins used: 10
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Xplor file |
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Refinement | *PLUS Lowest resolution: 27 Å / Rfactor Rfree: 0.329 / Rfactor Rwork: 0.208 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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