Text: THE STRUCTURE WAS DETERMINED FROM 2D HOMONUCLEAR NMR EXPERIMENTS USING UNLABELLED SAMPLE
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試料調製
詳細
溶媒系: 100% D2O OR 90% H2O, 10% D2O
試料状態
イオン強度: 100 mM KCL / pH: 7 / 圧: 1 atm / 温度: 303 K
結晶化
*PLUS
手法: other / 詳細: NMR
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NMR測定
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M
放射波長
相対比: 1
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Varian UNITY
Varian
UNITY
500
1
Varian UNITYPLUS
Varian
UNITYPLUS
600
2
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解析
NMR software
名称
バージョン
開発者
分類
Amber
7
CORNELL, W.D., ETAL.
精密化
Felix
95
Biosym
解析
NUCFIT
Lane, A.N.
データ解析
pfit
Conte, M.R., Bauer, C.J., Lane, A.N.
データ解析
精密化
手法: MULTIPLE STARTING CONFORMATIONS / ソフトェア番号: 1 詳細: DISTANCE CONSTRAINTS FOR GLYCOSIDIC TORSION ANGLES, PENTOSE PSEUDOROTATIONAL PHASE ANGLES/AMPLITUDES AND FRACTION OF SOUTH STATE SUGARS CALCULATED FROM NOE BUILD-UP CURVES AND COUPLING ...詳細: DISTANCE CONSTRAINTS FOR GLYCOSIDIC TORSION ANGLES, PENTOSE PSEUDOROTATIONAL PHASE ANGLES/AMPLITUDES AND FRACTION OF SOUTH STATE SUGARS CALCULATED FROM NOE BUILD-UP CURVES AND COUPLING CONSTANTS BY A LEAST-SQUARES/GRID SEARCH METHOD IMPLEMENTED IN NUCFIT AND PFIT (A.N. LANE, NIMR, UK). TEN DIFFERENT SETS OF UNIQUE RESTRAINTS GENERATED 10 STRUCTURES, THE AVERAGE OF WHICH SATISFIED THE EXPERIMENTALLY DERIVED FRACTION OF SOUTH STATE SUGARS BETTER THAN STRUCTURES GENERATED FROM A SINGLE SET OF RESTRAINTS. INTERNUCLEOTIDE DISTANCE CONSTRAINTS CALCULATED FROM NOE-BUILD-UP CURVES. STRUCTURES CALCULATED BY SIMULATED ANNEALING/RMD PROTOCOL WITHIN DISCOVER 95.0 USING AN AMBER FORCE FIELD AND A DISTANCE DIELECTRIC CONSTANT.
代表構造
選択基準: minimized average structure
NMRアンサンブル
コンフォーマー選択の基準: all calculated structures submitted 計算したコンフォーマーの数: 4 / 登録したコンフォーマーの数: 4