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- PDB-1og1: CRYSTAL STRUCTURE OF THE EUCARYOTIC MONO-ADP-RIBOSYLTRANSFERASE A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1og1 | ||||||
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Title | CRYSTAL STRUCTURE OF THE EUCARYOTIC MONO-ADP-RIBOSYLTRANSFERASE ART2.2 IN COMPLEX WITH TAD | ||||||
![]() | T-CELL ECTO-ADP-RIBOSYLTRANSFERASE 2 | ||||||
![]() | TRANSFERASE / ADP-RIBOSYLTRANSFERASE / IMMUNO-REGULATION | ||||||
Function / homology | ![]() NAD+ glycohydrolase / NAD+-protein-arginine ADP-ribosyltransferase / NAD+-protein-arginine ADP-ribosyltransferase activity / NAD catabolic process / NAD+ nucleosidase activity / hydrolase activity, acting on glycosyl bonds / NAD+ nucleotidase, cyclic ADP-ribose generating / NAD+-protein poly-ADP-ribosyltransferase activity / side of membrane / nucleotidyltransferase activity / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Ritter, H. / Koch-Nolte, F. / Marquez, V.E. / Schulz, G.E. | ||||||
![]() | ![]() Title: Substrate Binding and Catalysis of Ecto-Adp-Ribosyltransferase 2.2 From Rat Authors: Ritter, H. / Koch-Nolte, F. / Marquez, V.E. / Schulz, G.E. | ||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: AUTHOR PROVIDED. | ||||||
Remark 700 | SHEET DETERMINATION METHOD: AUTHOR PROVIDED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 63.6 KB | Display | ![]() |
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PDB format | ![]() | 46 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 805.3 KB | Display | ![]() |
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Full document | ![]() | 810.2 KB | Display | |
Data in XML | ![]() | 13.7 KB | Display | |
Data in CIF | ![]() | 19.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1og3C ![]() 1og4C ![]() 1gy0S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 26068.447 Da / Num. of mol.: 1 / Fragment: RESIDUES 21-246 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P20974, NAD+-protein-arginine ADP-ribosyltransferase |
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#2: Chemical | ChemComp-TAD / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.562 Å3/Da / Density % sol: 51 % |
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Crystal grow | pH: 8.3 Details: 100 MM TRIS PH8.3, 200 MM LI2SO4, 22 % PEG4000, pH 8.30 |
Crystal grow | *PLUS Method: other / Details: Mueller-Dieckmann., (2002) Acta Cryst., D58, 1211. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Sep 3, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.802 Å / Relative weight: 1 |
Reflection | Resolution: 2→20 Å / Num. obs: 20469 / % possible obs: 99.7 % / Redundancy: 4.4 % / Biso Wilson estimate: 32.2 Å2 / Rmerge(I) obs: 0.08 / Net I/σ(I): 20.3 |
Reflection shell | Resolution: 2→2.02 Å / Redundancy: 4.3 % / Rmerge(I) obs: 0.4 / Mean I/σ(I) obs: 2.8 / % possible all: 100 |
Reflection | *PLUS Highest resolution: 2 Å / Lowest resolution: 20 Å / Redundancy: 4.4 % / Rmerge(I) obs: 0.08 |
Reflection shell | *PLUS % possible obs: 100 % / Redundancy: 4.3 % / Num. unique obs: 698 / Rmerge(I) obs: 0.404 / Mean I/σ(I) obs: 2.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1GY0 Resolution: 2→20 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 1391101.46 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 54.5311 Å2 / ksol: 0.401936 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.7 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.13 Å / Rfactor Rfree error: 0.023 / Total num. of bins used: 6
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Xplor file | Serial no: 1 / Param file: MET1.PAR / Topol file: MET1.TOP | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 20 Å / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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