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- PDB-1gxy: crystal structure of the eucaryotic mono-ADP-ribosyltransferase A... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1gxy | ||||||
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Title | crystal structure of the eucaryotic mono-ADP-ribosyltransferase ART2.2; CRYSTAL FORM A (P21) | ||||||
![]() | T-CELL ECTO-ADP-RIBOSYLTRANSFERASE 2 | ||||||
![]() | TRANSFERASE / ADP-RIBOSYLTRANSFERASE / IMMUNO-REGULATION | ||||||
Function / homology | ![]() NAD+ glycohydrolase / NAD+-protein-arginine ADP-ribosyltransferase / NAD+-protein-arginine ADP-ribosyltransferase activity / NAD catabolic process / NAD+ nucleosidase activity / hydrolase activity, acting on glycosyl bonds / NAD+ nucleotidase, cyclic ADP-ribose generating / NAD+-protein poly-ADP-ribosyltransferase activity / side of membrane / nucleotidyltransferase activity / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mueller-Dieckmann, C. / Schulz, G.E. | ||||||
![]() | ![]() Title: Structure of the Ecto-Adp-Ribosyl Transferase Art2.2 From Rat Authors: Mueller-Dieckmann, C. / Ritter, H. / Haag, F. / Koch-Nolte, F. / Schulz, G.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 108.3 KB | Display | ![]() |
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PDB format | ![]() | 88.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 449.9 KB | Display | ![]() |
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Full document | ![]() | 453.5 KB | Display | |
Data in XML | ![]() | 23.8 KB | Display | |
Data in CIF | ![]() | 35.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (-0.12554, 0.02431, -0.99179), Vector: |
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Components
#1: Protein | Mass: 26068.447 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P20974, NAD+-protein-arginine ADP-ribosyltransferase #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 48 % | |||||||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.3 Details: 100 MM TRIS PH8.3, 200 MM LI2SO4, 22 % PEG4000, pH 8.30 | |||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 293 K / Method: vapor diffusion, hanging dropDetails: Mueller-Dieckmann, C., (2002) Acta Crystallogr., D58, 1211. | |||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9094 Å / Relative weight: 1 |
Reflection | Resolution: 1.71→29 Å / Num. obs: 48705 / % possible obs: 99.1 % / Redundancy: 4 % / Biso Wilson estimate: 17.5 Å2 / Rsym value: 0.034 / Net I/σ(I): 10.5 |
Reflection shell | Resolution: 1.71→1.8 Å / Redundancy: 3.8 % / Mean I/σ(I) obs: 6 / Rsym value: 0.119 / % possible all: 99.1 |
Reflection | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 29 Å / Num. measured all: 195638 / Rmerge(I) obs: 0.034 |
Reflection shell | *PLUS % possible obs: 99.1 % / Num. unique obs: 6787 / Rmerge(I) obs: 0.119 |
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Processing
Software | Name: CNS / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 50.77 Å2 / ksol: 0.36 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.71→29 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.71→1.82 Å / Rfactor Rfree error: 0.014 / Total num. of bins used: 6
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Refinement | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 35 Å / % reflection Rfree: 5 % / Rfactor Rfree: 0.223 / Rfactor Rwork: 0.19 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 21.1 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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