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Open data
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Basic information
Entry | Database: PDB / ID: 1oa8 | ||||||
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Title | AXH domain of human spinocerebellar ataxin-1 | ||||||
![]() | ATAXIN-1 | ||||||
![]() | RNA BINDING / HIGH MOBILITY GROUP HOMOLOGY / HMG / RNA-BINDING / DIMERIZATION | ||||||
Function / homology | ![]() poly(G) binding / nuclear inclusion body / nuclear export / poly(U) RNA binding / social behavior / RNA processing / learning / brain development / memory / nuclear matrix ...poly(G) binding / nuclear inclusion body / nuclear export / poly(U) RNA binding / social behavior / RNA processing / learning / brain development / memory / nuclear matrix / : / nervous system development / negative regulation of DNA-templated transcription / nucleolus / negative regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / identical protein binding / nucleus / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Allen, M.D. / Chen, Y.W. / Bycroft, M. | ||||||
![]() | ![]() Title: The structure of the AXH domain of spinocerebellar ataxin-1. Authors: Chen, Y.W. / Allen, M.D. / Veprintsev, D.B. / Lowe, J. / Bycroft, M. #1: Journal: FEBS Lett. / Year: 2003 Title: The Axh Module: An Independently Folded Domain Common to Ataxin-1 and Hbp1. Authors: De Chiara, C. / Giannini, C. / Adinolfi, S. / De Boer, J. / Guida, S. / Ramos, A. / Jodice, C. / Kioussis, D. / Pastore, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 117.4 KB | Display | ![]() |
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PDB format | ![]() | 91.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 381.9 KB | Display | ![]() |
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Full document | ![]() | 388.4 KB | Display | |
Data in XML | ![]() | 10.8 KB | Display | |
Data in CIF | ![]() | 19.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 14428.339 Da / Num. of mol.: 4 / Fragment: AXH DOMAIN, RESIDUES 562-694 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Compound details | ATAXIN-1 BINDS TO RNA IN VITRO. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.37 Å3/Da / Density % sol: 40 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 7 Details: PROTEIN WAS CRYSTALLIZED FROM 0.1M TRIS/HCL, PH 7.0, 0.2M AMMONIUM SULPHATE, 5 MM DTT, 15% PEG 4000, 20% GLYCEROL, AT 290K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 290 K / pH: 7 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
Detector | Type: ADSC CCD / Detector: CCD / Date: Nov 15, 2002 / Details: MIRRORS | ||||||||||||
Radiation | Monochromator: SI(111) / Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Resolution: 1.7→70.71 Å / Num. obs: 62044 / % possible obs: 99.6 % / Redundancy: 4.3 % / Rmerge(I) obs: 0.065 / Net I/σ(I): 5.9 | ||||||||||||
Reflection shell | Resolution: 1.7→1.79 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.416 / Mean I/σ(I) obs: 1.7 / % possible all: 98.1 | ||||||||||||
Reflection | *PLUS Highest resolution: 1.7 Å / Lowest resolution: 70.7 Å / Redundancy: 4.3 % / Num. measured all: 267655 / Rmerge(I) obs: 0.065 | ||||||||||||
Reflection shell | *PLUS % possible obs: 98.1 % / Redundancy: 3.4 % / Rmerge(I) obs: 0.416 / Mean I/σ(I) obs: 1.7 |
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Processing
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Refinement | Method to determine structure: ![]() Details: DISORDERED REGION IN B CHAIN (RESIDUES 39 - 51) MODELED BUILT FROM SE-METHONINE DATA.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL PLUS MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.8 Å2
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Refinement step | Cycle: LAST / Resolution: 1.7→70.71 Å
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Refine LS restraints |
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