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- PDB-1o70: Novel Fold Revealed by the Structure of a FAS1 Domain Pair from t... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1o70 | |||||||||
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Title | Novel Fold Revealed by the Structure of a FAS1 Domain Pair from the Insect Cell Adhesion Molecule Fasciclin I | |||||||||
![]() | FASCICLIN I | |||||||||
![]() | CELL ADHESION / AXON GUIDANCE / EXTRACELLULAR MODULE / GENETIC DISORDER / CORNEAL DYSTROPHY | |||||||||
Function / homology | ![]() calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules / neuron recognition / : / homophilic cell adhesion via plasma membrane adhesion molecules / cell adhesion molecule binding / extracellular matrix / extracellular matrix organization / axon guidance / cell adhesion / extracellular space / plasma membrane Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Clout, N.J. / Tisi, D. / Hohenester, E. | |||||||||
![]() | ![]() Title: Novel Fold Revealed by the Structure of a Fas1 Domain Pair from the Insect Cell Adhesion Molecule Fasciclin I Authors: Clout, N.J. / Tisi, D. / Hohenester, E. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 71.8 KB | Display | ![]() |
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PDB format | ![]() | 56.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 36493.141 Da / Num. of mol.: 1 / Fragment: FAS I DOMAINS 3 AND 4, RESIDUES 314-628 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
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#2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose Source method: isolated from a genetically manipulated source | ||||
#3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | Compound details | NEURAL CELL ADHESION MOLECULE THAT IS EXPRESSED ON DIFFERENT SUBSETS OF AXON BUNDLES IN INSECT EMBRYOS. | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.07 Å3/Da / Density % sol: 68 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.5 / Details: 0.1 M TRIS-HCL PH 8.5, 2.0 M AMMONIUM SULFATE | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 7.5 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: May 15, 2002 / Details: RH COATED SI MIRROR |
Radiation | Monochromator: A TRIANGULAR SINGLE CRYSTAL SI MONOCHROMATOR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.87 Å / Relative weight: 1 |
Reflection | Resolution: 2.6→20 Å / Num. obs: 17949 / % possible obs: 97.1 % / Redundancy: 8.4 % / Rmerge(I) obs: 0.058 / Net I/σ(I): 4.4 |
Reflection shell | Resolution: 2.6→20 Å / Redundancy: 8.3 % / Rmerge(I) obs: 0.31 / Mean I/σ(I) obs: 2.4 / % possible all: 97.5 |
Reflection | *PLUS Lowest resolution: 20 Å |
Reflection shell | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 2.74 Å / % possible obs: 97.5 % / Rmerge(I) obs: 0.309 |
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Processing
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Refinement | Method to determine structure: ![]()
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Displacement parameters | Biso mean: 32.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.6→20 Å
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Refine LS restraints |
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Xplor file |
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Refinement | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 20 Å / Num. reflection Rfree: 1758 / Rfactor Rfree: 0.263 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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