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- PDB-1ns2: Crystal structure of galactose mutarotase from Lactococcus lactis... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ns2 | ||||||
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Title | Crystal structure of galactose mutarotase from Lactococcus lactis mutant E304A complexed with galactose | ||||||
![]() | GALACTOSE MUTAROTASE | ||||||
![]() | ISOMERASE / MUTAROTASE / EPIMERASE / GALACTOSE METABOLISM | ||||||
Function / homology | ![]() aldose 1-epimerase / aldose 1-epimerase activity / galactose catabolic process via UDP-galactose / glucose metabolic process / carbohydrate binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Holden, H.M. / Thoden, J.B. | ||||||
![]() | ![]() Title: The Catalytic Mechanism of Galactose Mutarotase Authors: Thoden, J.B. / Kim, J. / Raushel, F.M. / Holden, H.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156 KB | Display | ![]() |
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PDB format | ![]() | 120.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 1ns0C ![]() 1ns4C ![]() 1ns7C ![]() 1ns8C ![]() 1nsmC ![]() 1nsrC ![]() 1nssC ![]() 1nsuC ![]() 1nsvC ![]() 1nsxC ![]() 1nszC ![]() 1l7jS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38564.922 Da / Num. of mol.: 2 / Mutation: E304A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Plasmid: PET28 / Production host: ![]() ![]() #2: Sugar | #3: Chemical | ChemComp-NI / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.23 Å3/Da / Density % sol: 44.48 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6 Details: MES, methylether PEG-5000, NaCl, pH 6.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / pH: 6 / Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS HI-STAR / Detector: AREA DETECTOR / Date: Mar 25, 2002 / Details: GOEBEL MIRRORS |
Radiation | Monochromator: GOEBEL OPTICS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54178 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→30 Å / Num. all: 50827 / Num. obs: 50827 / % possible obs: 94.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.5 % / Rsym value: 0.0702 / Net I/σ(I): 10.5 |
Reflection shell | Resolution: 1.95→2.04 Å / Redundancy: 1.9 % / Mean I/σ(I) obs: 2.9 / Num. unique all: 5840 / Rsym value: 0.248 / % possible all: 86.8 |
Reflection | *PLUS Highest resolution: 1.95 Å / Lowest resolution: 30 Å / Rmerge(I) obs: 0.0702 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1L7J Resolution: 1.95→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: ENGH & HUBER
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Refinement step | Cycle: LAST / Resolution: 1.95→30 Å
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Refine LS restraints |
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Refinement | *PLUS Lowest resolution: 30 Å / Num. reflection obs: 45793 | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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