[English] 日本語
![](img/lk-miru.gif)
- PDB-1mn0: Crystal structure of galactose mutarotase from lactococcus lactis... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1mn0 | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of galactose mutarotase from lactococcus lactis complexed with D-xylose | ||||||
![]() | Aldose 1-epimerase | ||||||
![]() | ISOMERASE / epimerase / sugar binding / galactosemia | ||||||
Function / homology | ![]() aldose 1-epimerase / aldose 1-epimerase activity / galactose catabolic process via UDP-galactose / glucose metabolic process / carbohydrate binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Thoden, J.B. / Kim, J. / Raushel, F.M. / Holden, H.M. | ||||||
![]() | ![]() Title: Structural and kinetic studies of sugar binding to galactose mutarotase from Lactococcus lactis. Authors: Thoden, J.B. / Kim, J. / Raushel, F.M. / Holden, H.M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 155.7 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 121.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 1mmuC ![]() 1mmxC ![]() 1mmyC ![]() 1mmzC ![]() 1l7jS C: citing same article ( S: Starting model for refinement |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 38622.957 Da / Num. of mol.: 2 / Mutation: E2S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: GALM / Plasmid: pET28 / Production host: ![]() ![]() #2: Sugar | #3: Chemical | ChemComp-NI / | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.7 % | ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG-5000-OMe, MES, D-xylose, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Diffraction | Mean temperature: 277 K |
---|---|
Diffraction source | Source: ![]() |
Detector | Type: SIEMENS HI-STAR / Detector: AREA DETECTOR / Date: Feb 6, 2002 / Details: goebel optics |
Radiation | Monochromator: goebel optics / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→30 Å / Num. all: 55244 / Num. obs: 55244 / % possible obs: 94 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.6 % / Biso Wilson estimate: 10.2 Å2 / Rsym value: 0.072 |
Reflection shell | Resolution: 1.9→1.99 Å / Redundancy: 1.8 % / Mean I/σ(I) obs: 2.8 / Num. unique all: 6116 / Rsym value: 0.243 / % possible all: 84.3 |
Reflection | *PLUS Rmerge(I) obs: 0.072 |
Reflection shell | *PLUS % possible obs: 84.3 % / Num. unique obs: 6116 / Rmerge(I) obs: 0.243 |
-
Processing
Software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: PDB entry 1L7J Resolution: 1.9→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
| |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→30 Å
| |||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||
Software | *PLUS Name: TNT / Version: 5F / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
|