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- PDB-1mmy: Crystal structure of galactose mutarotase from Lactococcus lactis... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mmy | |||||||||
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Title | Crystal structure of galactose mutarotase from Lactococcus lactis complexed with D-quinovose | |||||||||
![]() | Aldose 1-epimerase | |||||||||
![]() | ISOMERASE / epimerase / sugar binding / galactosemia | |||||||||
Function / homology | ![]() aldose 1-epimerase / aldose 1-epimerase activity / galactose catabolic process via UDP-galactose / glucose metabolic process / carbohydrate binding / cytoplasm Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Thoden, J.B. / Kim, J. / Raushel, F.M. / Holden, H.M. | |||||||||
![]() | ![]() Title: Structural and kinetic studies of sugar binding to galactose mutarotase from Lactococcus lactis. Authors: Thoden, J.B. / Kim, J. / Raushel, F.M. / Holden, H.M. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156.8 KB | Display | ![]() |
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PDB format | ![]() | 121.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.9 KB | Display | ![]() |
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Full document | ![]() | 485 KB | Display | |
Data in XML | ![]() | 32.6 KB | Display | |
Data in CIF | ![]() | 48 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1mmuC ![]() 1mmxC ![]() 1mmzC ![]() 1mn0C ![]() 1l7jS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 38622.957 Da / Num. of mol.: 2 / Mutation: E2S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: GALM / Plasmid: pET28 / Production host: ![]() ![]() #2: Sugar | #3: Chemical | ChemComp-NA / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.37 % | ||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6 Details: PEG-5000-OMe, MES, D-quinovose, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K | ||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 277 K |
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Diffraction source | Source: ![]() |
Detector | Type: SIEMENS HI-STAR / Detector: AREA DETECTOR / Date: May 17, 2002 / Details: goebel optics |
Radiation | Monochromator: goebel optics / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→30 Å / Num. all: 60843 / Num. obs: 60843 / % possible obs: 96.4 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 2.2 % / Rsym value: 0.063 / Net I/σ(I): 12 |
Reflection shell | Resolution: 1.85→1.93 Å / Redundancy: 1.7 % / Mean I/σ(I) obs: 3 / Num. unique all: 6968 / Rsym value: 0.251 / % possible all: 94.1 |
Reflection | *PLUS Rmerge(I) obs: 0.063 |
Reflection shell | *PLUS % possible obs: 94.1 % / Num. unique obs: 6968 / Rmerge(I) obs: 0.251 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 1L7J Resolution: 1.85→30 Å / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.85→30 Å
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Refine LS restraints |
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Software | *PLUS Name: TNT / Version: 5F / Classification: refinement | |||||||||||||||||||||||||
Refinement | *PLUS | |||||||||||||||||||||||||
Solvent computation | *PLUS | |||||||||||||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||||||||||||
Refine LS restraints | *PLUS
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